An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.
A T helper cell type 1-mediated colitis develops in severe combined immunodeficient mice after transfer of CD45RB(high) CD4(+) T cells and can be prevented by cotransfer of the CD45RB(low) subset. The immune-suppressive activities of the CD45RB(low) T cell population can be reversed in vivo by admin...
| Main Authors: | , , , , |
|---|---|
| 格式: | Journal article |
| 語言: | English |
| 出版: |
1999
|
| _version_ | 1826285495225679872 |
|---|---|
| author | Asseman, C Mauze, S Leach, M Coffman, R Powrie, F |
| author_facet | Asseman, C Mauze, S Leach, M Coffman, R Powrie, F |
| author_sort | Asseman, C |
| collection | OXFORD |
| description | A T helper cell type 1-mediated colitis develops in severe combined immunodeficient mice after transfer of CD45RB(high) CD4(+) T cells and can be prevented by cotransfer of the CD45RB(low) subset. The immune-suppressive activities of the CD45RB(low) T cell population can be reversed in vivo by administration of an anti-transforming growth factor beta antibody. Here we show that interleukin (IL)-10 is an essential mediator of the regulatory functions of the CD45RB(low) population. This population isolated from IL-10-deficient (IL-10(-/-)) mice was unable to protect from colitis and when transferred alone to immune-deficient recipients induced colitis. Treatment with an anti-murine IL-10 receptor monoclonal antibody abrogated inhibition of colitis mediated by wild-type (WT) CD45RB(low) CD4(+) cells, suggesting that IL-10 was necessary for the effector function of the regulatory T cell population. Inhibition of colitis by WT regulatory T cells was not dependent on IL-10 production by progeny of the CD45RB(high) CD4(+) cells, as CD45RB(low) CD4(+) cells from WT mice were able to inhibit colitis induced by IL-10(-/-) CD45RB(high) CD4(+) cells. These findings provide the first clear evidence that IL-10 plays a nonredundant role in the functioning of regulatory T cells that control inflammatory responses towards intestinal antigens. |
| first_indexed | 2024-03-07T01:29:41Z |
| format | Journal article |
| id | oxford-uuid:932f52a8-af69-4d38-8c6b-ae9739943cf4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:29:41Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:932f52a8-af69-4d38-8c6b-ae9739943cf42022-03-26T23:30:28ZAn essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:932f52a8-af69-4d38-8c6b-ae9739943cf4EnglishSymplectic Elements at Oxford1999Asseman, CMauze, SLeach, MCoffman, RPowrie, FA T helper cell type 1-mediated colitis develops in severe combined immunodeficient mice after transfer of CD45RB(high) CD4(+) T cells and can be prevented by cotransfer of the CD45RB(low) subset. The immune-suppressive activities of the CD45RB(low) T cell population can be reversed in vivo by administration of an anti-transforming growth factor beta antibody. Here we show that interleukin (IL)-10 is an essential mediator of the regulatory functions of the CD45RB(low) population. This population isolated from IL-10-deficient (IL-10(-/-)) mice was unable to protect from colitis and when transferred alone to immune-deficient recipients induced colitis. Treatment with an anti-murine IL-10 receptor monoclonal antibody abrogated inhibition of colitis mediated by wild-type (WT) CD45RB(low) CD4(+) cells, suggesting that IL-10 was necessary for the effector function of the regulatory T cell population. Inhibition of colitis by WT regulatory T cells was not dependent on IL-10 production by progeny of the CD45RB(high) CD4(+) cells, as CD45RB(low) CD4(+) cells from WT mice were able to inhibit colitis induced by IL-10(-/-) CD45RB(high) CD4(+) cells. These findings provide the first clear evidence that IL-10 plays a nonredundant role in the functioning of regulatory T cells that control inflammatory responses towards intestinal antigens. |
| spellingShingle | Asseman, C Mauze, S Leach, M Coffman, R Powrie, F An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. |
| title | An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. |
| title_full | An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. |
| title_fullStr | An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. |
| title_full_unstemmed | An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. |
| title_short | An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. |
| title_sort | essential role for interleukin 10 in the function of regulatory t cells that inhibit intestinal inflammation |
| work_keys_str_mv | AT assemanc anessentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT mauzes anessentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT leachm anessentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT coffmanr anessentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT powrief anessentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT assemanc essentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT mauzes essentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT leachm essentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT coffmanr essentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation AT powrief essentialroleforinterleukin10inthefunctionofregulatorytcellsthatinhibitintestinalinflammation |