RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements
The CRISPR/Cas system in prokaryotes provides resistance against invading viruses and plasmids. Three distinct stages in the mechanism can be recognized. Initially, fragments of invader DNAare integrated as newspacers into the repetitive CRISPR locus. Subsequently, the CRISPR is transcribed and the...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1826285556841054208 |
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| author | Jore, M Brouns, S van der Oost, J |
| author_facet | Jore, M Brouns, S van der Oost, J |
| author_sort | Jore, M |
| collection | OXFORD |
| description | The CRISPR/Cas system in prokaryotes provides resistance against invading viruses and plasmids. Three distinct stages in the mechanism can be recognized. Initially, fragments of invader DNAare integrated as newspacers into the repetitive CRISPR locus. Subsequently, the CRISPR is transcribed and the transcript is cleaved by a Cas protein within the repeats, generating short RNAs (crRNAs) that contain the spacer sequence. Finally, crRNAs guide the Cas protein machinery to a complementary invader target, either DNAor RNA, resulting in inhibition of virus or plasmid proliferation. In this article, we discuss our current understanding of this fascinating adaptive and heritable defense system, and describe functional similarities and differences with RNAi in eukaryotes. © 2012 Cold Spring Harbor Laboratory Press; all rights reserved. |
| first_indexed | 2024-03-07T01:30:36Z |
| format | Journal article |
| id | oxford-uuid:9378c940-f97f-4736-b07b-7443aed99edb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:30:36Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:9378c940-f97f-4736-b07b-7443aed99edb2022-03-26T23:32:31ZRNA in defense: CRISPRs protect prokaryotes against mobile genetic elementsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9378c940-f97f-4736-b07b-7443aed99edbEnglishSymplectic Elements at Oxford2012Jore, MBrouns, Svan der Oost, JThe CRISPR/Cas system in prokaryotes provides resistance against invading viruses and plasmids. Three distinct stages in the mechanism can be recognized. Initially, fragments of invader DNAare integrated as newspacers into the repetitive CRISPR locus. Subsequently, the CRISPR is transcribed and the transcript is cleaved by a Cas protein within the repeats, generating short RNAs (crRNAs) that contain the spacer sequence. Finally, crRNAs guide the Cas protein machinery to a complementary invader target, either DNAor RNA, resulting in inhibition of virus or plasmid proliferation. In this article, we discuss our current understanding of this fascinating adaptive and heritable defense system, and describe functional similarities and differences with RNAi in eukaryotes. © 2012 Cold Spring Harbor Laboratory Press; all rights reserved. |
| spellingShingle | Jore, M Brouns, S van der Oost, J RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements |
| title | RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements |
| title_full | RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements |
| title_fullStr | RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements |
| title_full_unstemmed | RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements |
| title_short | RNA in defense: CRISPRs protect prokaryotes against mobile genetic elements |
| title_sort | rna in defense crisprs protect prokaryotes against mobile genetic elements |
| work_keys_str_mv | AT jorem rnaindefensecrisprsprotectprokaryotesagainstmobilegeneticelements AT brounss rnaindefensecrisprsprotectprokaryotesagainstmobilegeneticelements AT vanderoostj rnaindefensecrisprsprotectprokaryotesagainstmobilegeneticelements |