Long-term stability of large insert genomic DNA episomal shuttle vectors in human cells.
We have constructed an episomal shuttle vector which can transfer large (>100 kb) human genomic DNA inserts back and forth between bacteria and human cells and which can be tracked in rapidly dividing human cells using a live cell assay. The vector (p5170) is based on the F factor-derived bac...
Main Authors: | Wade-Martins, R, Frampton, J, James, MR |
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Format: | Journal article |
Language: | English |
Published: |
1999
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