Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes
<p>Synthetic hexynyl α-D-mannopyranoside and its α-1,6-linked disaccharide counterpart were fluorescently labelled through CuAAC click chemistry with 3-azido-7-hydroxycoumarin. The resulting triazolyl-coumarin adducts, which were amenable to analysis by TLC, HPLC and mass spectrometry, proved...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
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Elsevier
2016
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| _version_ | 1797082760607694848 |
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| author | Ivanova, I Nepogodiev, S Saalbach, G O'Neill, E Urbaniak, M Ferguson, M Gurcha, S Besra, G Field, R |
| author_facet | Ivanova, I Nepogodiev, S Saalbach, G O'Neill, E Urbaniak, M Ferguson, M Gurcha, S Besra, G Field, R |
| author_sort | Ivanova, I |
| collection | OXFORD |
| description | <p>Synthetic hexynyl α-D-mannopyranoside and its α-1,6-linked disaccharide counterpart were fluorescently labelled through CuAAC click chemistry with 3-azido-7-hydroxycoumarin. The resulting triazolyl-coumarin adducts, which were amenable to analysis by TLC, HPLC and mass spectrometry, proved to be acceptor substrates for α-1,6-ManT activities in mycobacterial membranes, as well as α- and β-GalT activities in trypanosomal membranes, benchmarking the potential of the fluorescent acceptor approach against earlier radiochemical assays. Following on to explore the glycobiology of the benign protozoan alga Euglena gracilis, α-1,3- and α-1,2-ManT activities were detected in membrane preparations, along with GlcT, Glc-P-T and GlcNAc-P-T activities. These studies serve to demonstrate the potential of readily accessible fluorescent glycans as substrates for exploring carbohydrate active enzymes.</p> |
| first_indexed | 2024-03-07T01:32:24Z |
| format | Journal article |
| id | oxford-uuid:9409125d-35d2-4e61-854a-f07feb8fd5b5 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T01:32:24Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:9409125d-35d2-4e61-854a-f07feb8fd5b52022-03-26T23:36:28ZFluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9409125d-35d2-4e61-854a-f07feb8fd5b5Symplectic Elements at OxfordElsevier2016Ivanova, INepogodiev, SSaalbach, GO'Neill, EUrbaniak, MFerguson, MGurcha, SBesra, GField, R<p>Synthetic hexynyl α-D-mannopyranoside and its α-1,6-linked disaccharide counterpart were fluorescently labelled through CuAAC click chemistry with 3-azido-7-hydroxycoumarin. The resulting triazolyl-coumarin adducts, which were amenable to analysis by TLC, HPLC and mass spectrometry, proved to be acceptor substrates for α-1,6-ManT activities in mycobacterial membranes, as well as α- and β-GalT activities in trypanosomal membranes, benchmarking the potential of the fluorescent acceptor approach against earlier radiochemical assays. Following on to explore the glycobiology of the benign protozoan alga Euglena gracilis, α-1,3- and α-1,2-ManT activities were detected in membrane preparations, along with GlcT, Glc-P-T and GlcNAc-P-T activities. These studies serve to demonstrate the potential of readily accessible fluorescent glycans as substrates for exploring carbohydrate active enzymes.</p> |
| spellingShingle | Ivanova, I Nepogodiev, S Saalbach, G O'Neill, E Urbaniak, M Ferguson, M Gurcha, S Besra, G Field, R Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes |
| title | Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes |
| title_full | Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes |
| title_fullStr | Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes |
| title_full_unstemmed | Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes |
| title_short | Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes |
| title_sort | fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar 1 phosphate transferase activities in euglena gracilis membranes |
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