B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination
B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccin...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2022
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| _version_ | 1817932544312606720 |
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| author | Kotagiri, P Mescia, F Rae, WM Bergamaschi, L Tuong, ZK Turner, L Hunter, K Gerber, PP Hosmillo, M Hess, C Clatworthy, MR Goodfellow, IG Matheson, NJ McKinney, EF Wills, MR Gupta, RK Bradley, JR Bashford-Rogers, RJM Lyons, PA Smith, KGC |
| author2 | Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration |
| author_facet | Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration Kotagiri, P Mescia, F Rae, WM Bergamaschi, L Tuong, ZK Turner, L Hunter, K Gerber, PP Hosmillo, M Hess, C Clatworthy, MR Goodfellow, IG Matheson, NJ McKinney, EF Wills, MR Gupta, RK Bradley, JR Bashford-Rogers, RJM Lyons, PA Smith, KGC |
| author_sort | Kotagiri, P |
| collection | OXFORD |
| description | B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies. |
| first_indexed | 2024-12-09T03:39:36Z |
| format | Journal article |
| id | oxford-uuid:94355f38-fb08-41f9-b830-5841a3724cae |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:39:36Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:94355f38-fb08-41f9-b830-5841a3724cae2024-12-05T15:23:09ZB cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccinationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:94355f38-fb08-41f9-b830-5841a3724caeEnglishSymplectic ElementsElsevier2022Kotagiri, PMescia, FRae, WMBergamaschi, LTuong, ZKTurner, LHunter, KGerber, PPHosmillo, MHess, CClatworthy, MRGoodfellow, IGMatheson, NJMcKinney, EFWills, MRGupta, RKBradley, JRBashford-Rogers, RJMLyons, PASmith, KGCCambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource CollaborationB cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies. |
| spellingShingle | Kotagiri, P Mescia, F Rae, WM Bergamaschi, L Tuong, ZK Turner, L Hunter, K Gerber, PP Hosmillo, M Hess, C Clatworthy, MR Goodfellow, IG Matheson, NJ McKinney, EF Wills, MR Gupta, RK Bradley, JR Bashford-Rogers, RJM Lyons, PA Smith, KGC B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination |
| title | B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination |
| title_full | B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination |
| title_fullStr | B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination |
| title_full_unstemmed | B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination |
| title_short | B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination |
| title_sort | b cell receptor repertoire kinetics after sars cov 2 infection and vaccination |
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