Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration.
Direct intra-cerebral administration of substances into the brain parenchyma is a common technique used by researchers in neuroscience. However, inflammatory responses to the needle may confound the results obtained following injection of these substances. In this paper we show that the use of a gla...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1797082806866673664 |
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| author | McCluskey, L Campbell, S Anthony, D Allan, S |
| author_facet | McCluskey, L Campbell, S Anthony, D Allan, S |
| author_sort | McCluskey, L |
| collection | OXFORD |
| description | Direct intra-cerebral administration of substances into the brain parenchyma is a common technique used by researchers in neuroscience. However, inflammatory responses to the needle may confound the results obtained following injection of these substances. In this paper we show that the use of a glass micro-needle for intra-cerebral injection reduces mechanical injury, blood-brain barrier breakdown and neutrophil recruitment in response to the injection of vehicle or interleukin-1, compared to using a 26-gauge Hamilton syringe. Therefore, the use of a glass micro-needle to inject substances intra-cerebrally appears to cause minimal injection artefact and should be the method of choice. |
| first_indexed | 2024-03-07T01:33:04Z |
| format | Journal article |
| id | oxford-uuid:943de75a-8c52-479f-a794-0799d6d3d850 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:33:04Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:943de75a-8c52-479f-a794-0799d6d3d8502022-03-26T23:37:59ZInflammatory responses in the rat brain in response to different methods of intra-cerebral administration.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:943de75a-8c52-479f-a794-0799d6d3d850EnglishSymplectic Elements at Oxford2008McCluskey, LCampbell, SAnthony, DAllan, SDirect intra-cerebral administration of substances into the brain parenchyma is a common technique used by researchers in neuroscience. However, inflammatory responses to the needle may confound the results obtained following injection of these substances. In this paper we show that the use of a glass micro-needle for intra-cerebral injection reduces mechanical injury, blood-brain barrier breakdown and neutrophil recruitment in response to the injection of vehicle or interleukin-1, compared to using a 26-gauge Hamilton syringe. Therefore, the use of a glass micro-needle to inject substances intra-cerebrally appears to cause minimal injection artefact and should be the method of choice. |
| spellingShingle | McCluskey, L Campbell, S Anthony, D Allan, S Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration. |
| title | Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration. |
| title_full | Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration. |
| title_fullStr | Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration. |
| title_full_unstemmed | Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration. |
| title_short | Inflammatory responses in the rat brain in response to different methods of intra-cerebral administration. |
| title_sort | inflammatory responses in the rat brain in response to different methods of intra cerebral administration |
| work_keys_str_mv | AT mccluskeyl inflammatoryresponsesintheratbraininresponsetodifferentmethodsofintracerebraladministration AT campbells inflammatoryresponsesintheratbraininresponsetodifferentmethodsofintracerebraladministration AT anthonyd inflammatoryresponsesintheratbraininresponsetodifferentmethodsofintracerebraladministration AT allans inflammatoryresponsesintheratbraininresponsetodifferentmethodsofintracerebraladministration |