Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells.
We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targe...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Kluwer Academic Publishers
1992
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| _version_ | 1797082878783258624 |
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| author | Dorin, JR Dickinson, P Emslie, E Clarke, A Dobbie, L Hooper, M Halford, S Wainwright, B Porteous, D |
| author_facet | Dorin, JR Dickinson, P Emslie, E Clarke, A Dobbie, L Hooper, M Halford, S Wainwright, B Porteous, D |
| author_sort | Dorin, JR |
| collection | OXFORD |
| description | We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisely disrupts exon 10, the site of the major mutation in patients with cystic fibrosis. The targeted cells are pluripotent and competent to form chimaeras. |
| first_indexed | 2024-03-07T01:34:08Z |
| format | Journal article |
| id | oxford-uuid:94923638-b0e5-40b5-a1c3-e0cba2b1bde9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:34:08Z |
| publishDate | 1992 |
| publisher | Kluwer Academic Publishers |
| record_format | dspace |
| spelling | oxford-uuid:94923638-b0e5-40b5-a1c3-e0cba2b1bde92022-03-26T23:40:16ZSuccessful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:94923638-b0e5-40b5-a1c3-e0cba2b1bde9EnglishSymplectic Elements at OxfordKluwer Academic Publishers1992Dorin, JRDickinson, PEmslie, EClarke, ADobbie, LHooper, MHalford, SWainwright, BPorteous, DWe wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisely disrupts exon 10, the site of the major mutation in patients with cystic fibrosis. The targeted cells are pluripotent and competent to form chimaeras. |
| spellingShingle | Dorin, JR Dickinson, P Emslie, E Clarke, A Dobbie, L Hooper, M Halford, S Wainwright, B Porteous, D Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells. |
| title | Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells. |
| title_full | Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells. |
| title_fullStr | Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells. |
| title_full_unstemmed | Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells. |
| title_short | Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells. |
| title_sort | successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells |
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