GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY
In vivo 31P-nuclear magnetic resonance (NMR) spectroscopy was used to monitor the metabolism of d-galactose in excised root tissues from maize (Zea mays) seedlings. The accumulation of galactose 1-phosphate and uridinediphosphogalactose (UDP-gal) in the tissue was correlated with changes in the gluc...
| Huvudupphovsmän: | , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
1988
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| _version_ | 1826285826788556800 |
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| author | Loughman, B Ratcliffe, R Schwabe, J |
| author_facet | Loughman, B Ratcliffe, R Schwabe, J |
| author_sort | Loughman, B |
| collection | OXFORD |
| description | In vivo 31P-nuclear magnetic resonance (NMR) spectroscopy was used to monitor the metabolism of d-galactose in excised root tissues from maize (Zea mays) seedlings. The accumulation of galactose 1-phosphate and uridinediphosphogalactose (UDP-gal) in the tissue was correlated with changes in the glucose 6-phosphate, cytoplasmic Pi, vacuolar Pi and NTP levels. NMR revealed a complicated time dependence for these metabolites with the galactose 1-phosphate signal going through a maximum after the NTP level had gone through a minimum. Many of the spectroscopic changes could be understood by viewing galactose as a sink for Pi and by supplying external Pi it was possible to modify the tissue response to galactose. Increasing the external galactose concentration increased the ratio of galactose 1-phosphate to UDP-gal in the loaded tissue whereas the protein synthesis inhibitor cycloheximide, which caused a marked increase in the NTP signals, reduced the ratio. The results suggest that the assimilation of galactose is limited by the slow metabolism of UDP-gal. © 1989. |
| first_indexed | 2024-03-07T01:34:39Z |
| format | Journal article |
| id | oxford-uuid:94b97e9c-01b1-4857-a95e-59fa0a1bbc0b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:34:39Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:94b97e9c-01b1-4857-a95e-59fa0a1bbc0b2022-03-26T23:41:28ZGALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPYJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:94b97e9c-01b1-4857-a95e-59fa0a1bbc0bEnglishSymplectic Elements at Oxford1988Loughman, BRatcliffe, RSchwabe, JIn vivo 31P-nuclear magnetic resonance (NMR) spectroscopy was used to monitor the metabolism of d-galactose in excised root tissues from maize (Zea mays) seedlings. The accumulation of galactose 1-phosphate and uridinediphosphogalactose (UDP-gal) in the tissue was correlated with changes in the glucose 6-phosphate, cytoplasmic Pi, vacuolar Pi and NTP levels. NMR revealed a complicated time dependence for these metabolites with the galactose 1-phosphate signal going through a maximum after the NTP level had gone through a minimum. Many of the spectroscopic changes could be understood by viewing galactose as a sink for Pi and by supplying external Pi it was possible to modify the tissue response to galactose. Increasing the external galactose concentration increased the ratio of galactose 1-phosphate to UDP-gal in the loaded tissue whereas the protein synthesis inhibitor cycloheximide, which caused a marked increase in the NTP signals, reduced the ratio. The results suggest that the assimilation of galactose is limited by the slow metabolism of UDP-gal. © 1989. |
| spellingShingle | Loughman, B Ratcliffe, R Schwabe, J GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY |
| title | GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY |
| title_full | GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY |
| title_fullStr | GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY |
| title_full_unstemmed | GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY |
| title_short | GALACTOSE METABOLISM IN ZEA-MAYS ROOT TISSUES OBSERVED BY P-31-NMR SPECTROSCOPY |
| title_sort | galactose metabolism in zea mays root tissues observed by p 31 nmr spectroscopy |
| work_keys_str_mv | AT loughmanb galactosemetabolisminzeamaysroottissuesobservedbyp31nmrspectroscopy AT ratcliffer galactosemetabolisminzeamaysroottissuesobservedbyp31nmrspectroscopy AT schwabej galactosemetabolisminzeamaysroottissuesobservedbyp31nmrspectroscopy |