Tick histamine-binding proteins: lipocalins with a second binding cavity.
Tick histamine-binding proteins (HBPs) are lipocalins with two binding pockets. One of these binds histamine with a high affinity and is found at the position expected from other lipocalins, adjacent to the omega-loop at the open-end of the beta-barrel. A second binding cavity, which is a low-affini...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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| _version_ | 1797082917224054784 |
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| author | Paesen, G Adams, P Nuttall, P Stuart, D |
| author_facet | Paesen, G Adams, P Nuttall, P Stuart, D |
| author_sort | Paesen, G |
| collection | OXFORD |
| description | Tick histamine-binding proteins (HBPs) are lipocalins with two binding pockets. One of these binds histamine with a high affinity and is found at the position expected from other lipocalins, adjacent to the omega-loop at the open-end of the beta-barrel. A second binding cavity, which is a low-affinity site for histamine in one of the HBPs, is located at the end of the barrel that is closed off in other lipocalins. In order to create the second site, the 'closed-end' region has undergone a major reconstruction. Typical lipocalin characteristics, such as the 3(10) helix and a structural cluster of highly conserved residues, have been lost, while an alpha-helix now shields the cavity from the exterior. The prominence of acidic residues in the binding pockets is another distinctive characteristic of HBPs. Whereas most lipocalins have highly hydrophobic binding cavities designed to bind lipophilic compounds, HBPs have evolved to trap cationic, hydrophilic molecules. |
| first_indexed | 2024-03-07T01:34:42Z |
| format | Journal article |
| id | oxford-uuid:94bf1af2-1efb-445a-a691-d862d9efaaf2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:34:42Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:94bf1af2-1efb-445a-a691-d862d9efaaf22022-03-26T23:41:36ZTick histamine-binding proteins: lipocalins with a second binding cavity.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:94bf1af2-1efb-445a-a691-d862d9efaaf2EnglishSymplectic Elements at Oxford2000Paesen, GAdams, PNuttall, PStuart, DTick histamine-binding proteins (HBPs) are lipocalins with two binding pockets. One of these binds histamine with a high affinity and is found at the position expected from other lipocalins, adjacent to the omega-loop at the open-end of the beta-barrel. A second binding cavity, which is a low-affinity site for histamine in one of the HBPs, is located at the end of the barrel that is closed off in other lipocalins. In order to create the second site, the 'closed-end' region has undergone a major reconstruction. Typical lipocalin characteristics, such as the 3(10) helix and a structural cluster of highly conserved residues, have been lost, while an alpha-helix now shields the cavity from the exterior. The prominence of acidic residues in the binding pockets is another distinctive characteristic of HBPs. Whereas most lipocalins have highly hydrophobic binding cavities designed to bind lipophilic compounds, HBPs have evolved to trap cationic, hydrophilic molecules. |
| spellingShingle | Paesen, G Adams, P Nuttall, P Stuart, D Tick histamine-binding proteins: lipocalins with a second binding cavity. |
| title | Tick histamine-binding proteins: lipocalins with a second binding cavity. |
| title_full | Tick histamine-binding proteins: lipocalins with a second binding cavity. |
| title_fullStr | Tick histamine-binding proteins: lipocalins with a second binding cavity. |
| title_full_unstemmed | Tick histamine-binding proteins: lipocalins with a second binding cavity. |
| title_short | Tick histamine-binding proteins: lipocalins with a second binding cavity. |
| title_sort | tick histamine binding proteins lipocalins with a second binding cavity |
| work_keys_str_mv | AT paeseng tickhistaminebindingproteinslipocalinswithasecondbindingcavity AT adamsp tickhistaminebindingproteinslipocalinswithasecondbindingcavity AT nuttallp tickhistaminebindingproteinslipocalinswithasecondbindingcavity AT stuartd tickhistaminebindingproteinslipocalinswithasecondbindingcavity |