Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart.
PURPOSE: Butyrate, a short chain fatty acid, was studied as a novel hyperpolarized substrate for use in dynamic nuclear polarization enhanced magnetic resonance spectroscopy experiments, to define the pathways of short chain fatty acid and ketone body metabolism in real time. METHODS: Butyrate was p...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Wiley
2014
|
| _version_ | 1826285840516513792 |
|---|---|
| author | Ball, DR Rowlands, B Dodd, M Le Page, L Ball, V Carr, C Clarke, K Tyler, D |
| author_facet | Ball, DR Rowlands, B Dodd, M Le Page, L Ball, V Carr, C Clarke, K Tyler, D |
| author_sort | Ball, DR |
| collection | OXFORD |
| description | PURPOSE: Butyrate, a short chain fatty acid, was studied as a novel hyperpolarized substrate for use in dynamic nuclear polarization enhanced magnetic resonance spectroscopy experiments, to define the pathways of short chain fatty acid and ketone body metabolism in real time. METHODS: Butyrate was polarized via the dynamic nuclear polarization process and subsequently dissolved to generate an injectable metabolic substrate. Metabolism was initially assessed in the isolated perfused rat heart, followed by evaluation in the in vivo rat heart. RESULTS: Hyperpolarized butyrate was generated with a polarization level of 7% and was shown to have a T1 relaxation time of 20 s. These physical characteristics were sufficient to enable assessment of multiple steps in its metabolism, with the ketone body acetoacetate and several tricarboxylic acid cycle intermediates observed both in vitro and in vivo. Metabolite to butyrate ratios of 0.1-0.4% and 0.5-2% were observed in vitro and in vivo respectively, similar to levels previously observed with hyperpolarized [2-(13) C]pyruvate. CONCLUSIONS: In this study, butyrate has been demonstrated to be a suitable hyperpolarized substrate capable of revealing multi-step metabolism in dynamic nuclear polarization experiments and providing information on the metabolism of fatty acids not currently achievable with other hyperpolarized substrates. |
| first_indexed | 2024-03-07T01:34:52Z |
| format | Journal article |
| id | oxford-uuid:94cb3034-92c9-4c3c-933b-f1f58321fe1f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:34:52Z |
| publishDate | 2014 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:94cb3034-92c9-4c3c-933b-f1f58321fe1f2022-03-26T23:41:55ZHyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:94cb3034-92c9-4c3c-933b-f1f58321fe1fEnglishSymplectic Elements at OxfordWiley2014Ball, DRRowlands, BDodd, MLe Page, LBall, VCarr, CClarke, KTyler, DPURPOSE: Butyrate, a short chain fatty acid, was studied as a novel hyperpolarized substrate for use in dynamic nuclear polarization enhanced magnetic resonance spectroscopy experiments, to define the pathways of short chain fatty acid and ketone body metabolism in real time. METHODS: Butyrate was polarized via the dynamic nuclear polarization process and subsequently dissolved to generate an injectable metabolic substrate. Metabolism was initially assessed in the isolated perfused rat heart, followed by evaluation in the in vivo rat heart. RESULTS: Hyperpolarized butyrate was generated with a polarization level of 7% and was shown to have a T1 relaxation time of 20 s. These physical characteristics were sufficient to enable assessment of multiple steps in its metabolism, with the ketone body acetoacetate and several tricarboxylic acid cycle intermediates observed both in vitro and in vivo. Metabolite to butyrate ratios of 0.1-0.4% and 0.5-2% were observed in vitro and in vivo respectively, similar to levels previously observed with hyperpolarized [2-(13) C]pyruvate. CONCLUSIONS: In this study, butyrate has been demonstrated to be a suitable hyperpolarized substrate capable of revealing multi-step metabolism in dynamic nuclear polarization experiments and providing information on the metabolism of fatty acids not currently achievable with other hyperpolarized substrates. |
| spellingShingle | Ball, DR Rowlands, B Dodd, M Le Page, L Ball, V Carr, C Clarke, K Tyler, D Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart. |
| title | Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart. |
| title_full | Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart. |
| title_fullStr | Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart. |
| title_full_unstemmed | Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart. |
| title_short | Hyperpolarized butyrate: a metabolic probe of short chain fatty acid metabolism in the heart. |
| title_sort | hyperpolarized butyrate a metabolic probe of short chain fatty acid metabolism in the heart |
| work_keys_str_mv | AT balldr hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT rowlandsb hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT doddm hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT lepagel hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT ballv hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT carrc hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT clarkek hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart AT tylerd hyperpolarizedbutyrateametabolicprobeofshortchainfattyacidmetabolismintheheart |