The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages
The translocator protein (TSPO) is a mitochondrial membrane protein, of as yet uncertain function. Its purported high expression on activated macrophages, has lent utility to TSPO targeted molecular imaging in the form of positron emission tomography (PET), as a means to detect and quantify inflamma...
| Những tác giả chính: | , , , , , , , , |
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
| Được phát hành: |
Plubic Library of Science
2017
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| Những chủ đề: |
| _version_ | 1826285928458485760 |
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| author | Narayan, N Mandhair, H Smyth, E Dakin, S Kiriakidis, S Wells, L Owen, D Sabokbar, A Taylor, P |
| author_facet | Narayan, N Mandhair, H Smyth, E Dakin, S Kiriakidis, S Wells, L Owen, D Sabokbar, A Taylor, P |
| author_sort | Narayan, N |
| collection | OXFORD |
| description | The translocator protein (TSPO) is a mitochondrial membrane protein, of as yet uncertain function. Its purported high expression on activated macrophages, has lent utility to TSPO targeted molecular imaging in the form of positron emission tomography (PET), as a means to detect and quantify inflammation in vivo. However, existing literature regarding TSPO expression on human activated macrophages is lacking, mostly deriving from brain tissue studies, including studies of brain malignancy, and inflammatory diseases such as multiple sclerosis. Here, we utilized three human sources of monocyte derived macrophages (MDM), from THP-1 monocytes, healthy peripheral blood monocytes and synovial fluid monocytes from patients with rheumatoid arthritis, to undertake a detailed investigation of TSPO expression in activated macrophages. In this work, we demonstrate a consistent down-regulation of TSPO mRNA and protein in macrophages activated to a pro-inflammatory, or 'M1' phenotype. Conversely, stimulation of macrophages to an M2 phenotype with IL-4, dexamethasone or TGF-β1 did not alter TSPO expression, regardless of MDM source. The reasons for this are uncertain, but our study findings add some supporting evidence for recent investigations concluding that TSPO may be involved in negative regulation of inflammatory responses in macrophages. |
| first_indexed | 2024-03-07T01:36:11Z |
| format | Journal article |
| id | oxford-uuid:95438dfe-4816-4ff0-a010-47b0396b673b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:36:11Z |
| publishDate | 2017 |
| publisher | Plubic Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:95438dfe-4816-4ff0-a010-47b0396b673b2022-03-26T23:45:03ZThe macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophagesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:95438dfe-4816-4ff0-a010-47b0396b673bSynovial fluidImmunofluorescence stainingProtein expressionacrophagesBloodPhenotypesIn vivo imagingMonocytesEnglishSymplectic Elements at OxfordPlubic Library of Science2017Narayan, NMandhair, HSmyth, EDakin, SKiriakidis, SWells, LOwen, DSabokbar, ATaylor, PThe translocator protein (TSPO) is a mitochondrial membrane protein, of as yet uncertain function. Its purported high expression on activated macrophages, has lent utility to TSPO targeted molecular imaging in the form of positron emission tomography (PET), as a means to detect and quantify inflammation in vivo. However, existing literature regarding TSPO expression on human activated macrophages is lacking, mostly deriving from brain tissue studies, including studies of brain malignancy, and inflammatory diseases such as multiple sclerosis. Here, we utilized three human sources of monocyte derived macrophages (MDM), from THP-1 monocytes, healthy peripheral blood monocytes and synovial fluid monocytes from patients with rheumatoid arthritis, to undertake a detailed investigation of TSPO expression in activated macrophages. In this work, we demonstrate a consistent down-regulation of TSPO mRNA and protein in macrophages activated to a pro-inflammatory, or 'M1' phenotype. Conversely, stimulation of macrophages to an M2 phenotype with IL-4, dexamethasone or TGF-β1 did not alter TSPO expression, regardless of MDM source. The reasons for this are uncertain, but our study findings add some supporting evidence for recent investigations concluding that TSPO may be involved in negative regulation of inflammatory responses in macrophages. |
| spellingShingle | Synovial fluid Immunofluorescence staining Protein expression acrophages Blood Phenotypes In vivo imaging Monocytes Narayan, N Mandhair, H Smyth, E Dakin, S Kiriakidis, S Wells, L Owen, D Sabokbar, A Taylor, P The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages |
| title | The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages |
| title_full | The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages |
| title_fullStr | The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages |
| title_full_unstemmed | The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages |
| title_short | The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages |
| title_sort | macrophage marker translocator protein tspo is down regulated on pro inflammatory m1 human macrophages |
| topic | Synovial fluid Immunofluorescence staining Protein expression acrophages Blood Phenotypes In vivo imaging Monocytes |
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