Reconstitution of the spliceosomal U1 snRNP from all recombinant subunits and its characterisation by ionspray Q-tof mass-spectrometry.
The first important step in pre-mRNA splicing is the recognition of the 5' splice site by the U1 snRNP. It consists of U1 snRNA and 10 protein subunits. We have reconstituted the U1 snRNP from all its ten proteins produced in E. coli and U1 snRNA transcribed in vitro. We have used nano spray ti...
| Autori principali: | , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
2001
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