Honing the double-edged sword: improving human iPSC-microglia models
Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial mode...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Frontiers Media
2020
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| _version_ | 1826286119124205568 |
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| author | Hedegaard, A Stodolak, S James, WS Cowley, SS |
| author_facet | Hedegaard, A Stodolak, S James, WS Cowley, SS |
| author_sort | Hedegaard, A |
| collection | OXFORD |
| description | Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the in vitro microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models. |
| first_indexed | 2024-03-07T01:39:02Z |
| format | Journal article |
| id | oxford-uuid:9630e9a8-7e83-4acf-a3e4-85f2c8a32517 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:39:02Z |
| publishDate | 2020 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:9630e9a8-7e83-4acf-a3e4-85f2c8a325172022-03-26T23:51:28ZHoning the double-edged sword: improving human iPSC-microglia modelsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9630e9a8-7e83-4acf-a3e4-85f2c8a32517EnglishSymplectic ElementsFrontiers Media2020Hedegaard, AStodolak, SJames, WSCowley, SSHuman induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the in vitro microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models. |
| spellingShingle | Hedegaard, A Stodolak, S James, WS Cowley, SS Honing the double-edged sword: improving human iPSC-microglia models |
| title | Honing the double-edged sword: improving human iPSC-microglia models |
| title_full | Honing the double-edged sword: improving human iPSC-microglia models |
| title_fullStr | Honing the double-edged sword: improving human iPSC-microglia models |
| title_full_unstemmed | Honing the double-edged sword: improving human iPSC-microglia models |
| title_short | Honing the double-edged sword: improving human iPSC-microglia models |
| title_sort | honing the double edged sword improving human ipsc microglia models |
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