Microscope-AOtools: A generalised adaptive optics implementation
Aberrations arising from sources such as sample heterogeneity and refractive index mismatches are constant problems in biological imaging. These aberrations reduce image quality and the achievable depth of imaging, particularly in super-resolution microscopy techniques. Adaptive optics (AO) technolo...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Optical Society of America
2020
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| _version_ | 1797083334589808640 |
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| author | Hall, N Titlow, J Booth, MJ Dobbie, IM |
| author_facet | Hall, N Titlow, J Booth, MJ Dobbie, IM |
| author_sort | Hall, N |
| collection | OXFORD |
| description | Aberrations arising from sources such as sample heterogeneity and refractive index mismatches are constant problems in biological imaging. These aberrations reduce image quality and the achievable depth of imaging, particularly in super-resolution microscopy techniques. Adaptive optics (AO) technology has been proven to be effective in correcting for these aberrations, thereby improving the image quality. However, it has not been widely adopted by the biological imaging community due, in part, to difficulty in set-up and operation of AO. The methods for doing so are not novel or unknown, but new users often waste time and effort reimplementing existing methods for their specific set-ups, hardware, sample types, etc. Microscope-AOtools offers a robust, easy-to-use implementation of the essential methods for set-up and use of AO elements and techniques. These methods are constructed in a generalised manner that can utilise a range of adaptive optics elements, wavefront sensing techniques and sensorless AO correction methods. Furthermore, the methods are designed to be easily extensible as new techniques arise, leading to a streamlined pipeline for new AO technology and techniques to be adopted by the wider microscopy community. |
| first_indexed | 2024-03-07T01:40:17Z |
| format | Journal article |
| id | oxford-uuid:969f7b60-a6e2-4e16-bbaf-007b4ebfc73c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:40:17Z |
| publishDate | 2020 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | oxford-uuid:969f7b60-a6e2-4e16-bbaf-007b4ebfc73c2022-03-26T23:54:12ZMicroscope-AOtools: A generalised adaptive optics implementationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:969f7b60-a6e2-4e16-bbaf-007b4ebfc73cEnglishSymplectic ElementsOptical Society of America2020Hall, NTitlow, JBooth, MJDobbie, IMAberrations arising from sources such as sample heterogeneity and refractive index mismatches are constant problems in biological imaging. These aberrations reduce image quality and the achievable depth of imaging, particularly in super-resolution microscopy techniques. Adaptive optics (AO) technology has been proven to be effective in correcting for these aberrations, thereby improving the image quality. However, it has not been widely adopted by the biological imaging community due, in part, to difficulty in set-up and operation of AO. The methods for doing so are not novel or unknown, but new users often waste time and effort reimplementing existing methods for their specific set-ups, hardware, sample types, etc. Microscope-AOtools offers a robust, easy-to-use implementation of the essential methods for set-up and use of AO elements and techniques. These methods are constructed in a generalised manner that can utilise a range of adaptive optics elements, wavefront sensing techniques and sensorless AO correction methods. Furthermore, the methods are designed to be easily extensible as new techniques arise, leading to a streamlined pipeline for new AO technology and techniques to be adopted by the wider microscopy community. |
| spellingShingle | Hall, N Titlow, J Booth, MJ Dobbie, IM Microscope-AOtools: A generalised adaptive optics implementation |
| title | Microscope-AOtools: A generalised adaptive optics implementation |
| title_full | Microscope-AOtools: A generalised adaptive optics implementation |
| title_fullStr | Microscope-AOtools: A generalised adaptive optics implementation |
| title_full_unstemmed | Microscope-AOtools: A generalised adaptive optics implementation |
| title_short | Microscope-AOtools: A generalised adaptive optics implementation |
| title_sort | microscope aotools a generalised adaptive optics implementation |
| work_keys_str_mv | AT halln microscopeaotoolsageneralisedadaptiveopticsimplementation AT titlowj microscopeaotoolsageneralisedadaptiveopticsimplementation AT boothmj microscopeaotoolsageneralisedadaptiveopticsimplementation AT dobbieim microscopeaotoolsageneralisedadaptiveopticsimplementation |