Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis.

Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis.

The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more specimens were PCR positive for 47 of 77 patients with c...

Full description

Bibliographic Details
Main Authors:	Chantratita, N, Wuthiekanun, V, Limmathurotsakul, D, Thanwisai, A, Chantratita, W, Day, N, Peacock, S
Format:	Journal article
Language:	English
Published:	2007

Similar Items

Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis.
by: Chantratita, N, et al.
Published: (2007)

Enzyme-linked immunosorbent assay for the diagnosis of melioidosis: better than we thought.
by: Limmathurotsakul, D, et al.
Published: (2011)

Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis.
by: Chantratita, N, et al.
Published: (2008)

Simultaneous infection with more than one strain of Burkholderia pseudomallei is uncommon in human melioidosis.
by: Limmathurotsakul, D, et al.
Published: (2007)

Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipL32 genes for human leptospirosis in Thailand: a case-control study.
by: Thaipadungpanit, J, et al.
Published: (2011)

Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipL32 genes for human leptospirosis in Thailand: a case-control study.
by: Janjira Thaipadungpanit, et al.
Published: (2011-01-01)

Role of Burkholderia pseudomallei biofilm formation and lipopolysaccharide in relapse of melioidosis.
by: Limmathurotsakul, D, et al.
Published: (2014)

A simple scoring system to differentiate between relapse and re-infection in patients with recurrent melioidosis
by: Limmathurotsakul, D, et al.
Published: (2008)

Optimization and prospective evaluation of sensitive real-time PCR assays with an internal control for the diagnosis of melioidosis in Thailand
by: Chawitar Noparatvarakorn, et al.
Published: (2023-12-01)

Toll-like receptor 4 region genetic variants are associated with susceptibility to melioidosis.
by: West, T, et al.
Published: (2012)

Impaired TLR5 functionality is associated with survival in melioidosis.
by: West, T, et al.
Published: (2013)

The role of NOD2 in murine and human melioidosis.
by: Myers, N, et al.
Published: (2014)

Antibodies in melioidosis: the role of the indirect hemagglutination assay in evaluating patients and exposed populations
by: Chaichana, P, et al.
Published: (2018)

Common TLR1 genetic variation is not associated with death from melioidosis, a common cause of sepsis in rural Thailand
by: Chantratita, N, et al.
Published: (2014)

Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay.
by: Chantratita, N, et al.
Published: (2013)

Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro
by: Tandhavanant, S, et al.
Published: (2010)

Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models.
by: Limmathurotsakul, D, et al.
Published: (2010)

Recurrent melioidosis in patients in northeast Thailand is frequently due to reinfection rather than relapse.
by: Maharjan, B, et al.
Published: (2005)

Melioidosis caused by Burkholderia pseudomallei in drinking water, Thailand, 2012.
by: Limmathurotsakul, D, et al.
Published: (2014)

Burkholderia Pseudomallei is genetically diverse in agricultural land in Northeast Thailand
by: Wuthiekanun, V, et al.
Published: (2009)

A simple scoring system to differentiate between relapse and re-infection in patients with recurrent melioidosis.
by: Direk Limmathurotsakul, et al.
Published: (2008-01-01)

T-cell responses are associated with survival in acute Melioidosis patients
by: Dunachie, S, et al.
Published: (2015)

Genetic diversity and microevolution of Burkholderia pseudomallei in the environment.
by: Chantratita, N, et al.
Published: (2008)

Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay.
by: Tiyawisutsri, R, et al.
Published: (2005)

Survival of Burkholderia pseudomallei in distilled water for 16 years
by: Pumpuang, A, et al.
Published: (2011)

Survival of Burkholderia pseudomallei in distilled water for 16 years.
by: Pumpuang, A, et al.
Published: (2011)

Patterns of organ involvement in recurrent melioidosis.
by: Limmathurotsakul, D, et al.
Published: (2009)

Risk factors for recurrent melioidosis in northeast Thailand.
by: Limmathurotsakul, D, et al.
Published: (2006)

Burkholderia pseudomallei is spatially distributed in soil in northeast Thailand
by: Limmathurotsakul, D, et al.
Published: (2010)

Prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast Thailand.
by: Cheng, A, et al.
Published: (2006)

Intensity of exposure and incidence of melioidosis in Thai children.
by: Cheng, A, et al.
Published: (2008)

Role and significance of quantitative urine cultures in diagnosis of melioidosis.
by: Limmathurotsakul, D, et al.
Published: (2005)

Gastrointestinal tract involvement in melioidosis.
by: Teparrukkul, P, et al.
Published: (2017)

Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of Burkholderia pseudomallei in clinical specimens.
by: Tandhavanant, S, et al.
Published: (2013)

Short Report: Patterns of Organ Involvement in Recurrent Melioidosis
by: Limmathurotsakul, D, et al.
Published: (2009)

Rapid immunofluorescence microscopy for diagnosis of melioidosis.
by: Wuthiekanun, V, et al.
Published: (2005)

A matched case-control study identifies activities of daily living associated with acquisition of melioidosis in northeast Thailand
by: Limmathurotsakul, D, et al.
Published: (2012)

Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models.
by: Direk Limmathurotsakul, et al.
Published: (2010-08-01)

Activities of daily living associated with acquisition of melioidosis in northeast Thailand: a matched case-control study
by: Limmathurotsakul, D, et al.
Published: (2013)

Fatal melioidosis in goats in Bangkok, Thailand.
by: Tonpitak, W, et al.
Published: (2014)