Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells.
Dendritic cells (DCs) are able to present glycolipids to invariant natural killer T (iNKT) cells in vivo. Very few compounds have been found to stimulate iNKT cells, and of these, the best characterised is the glycolipid α-galactosylceramide, which stimulates the production of large quantities of in...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1826286351464529920 |
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| author | Kaur, R Chen, J Dawoodji, A Cerundolo, V Garcia-Diaz, Y Wojno, J Cox, L Besra, G Moghaddam, B Perrie, Y |
| author_facet | Kaur, R Chen, J Dawoodji, A Cerundolo, V Garcia-Diaz, Y Wojno, J Cox, L Besra, G Moghaddam, B Perrie, Y |
| author_sort | Kaur, R |
| collection | OXFORD |
| description | Dendritic cells (DCs) are able to present glycolipids to invariant natural killer T (iNKT) cells in vivo. Very few compounds have been found to stimulate iNKT cells, and of these, the best characterised is the glycolipid α-galactosylceramide, which stimulates the production of large quantities of interferon-gamma (IFN-γ) and interleukin-4 (IL-4). However, αGalCer leads to overstimulation of iNKT cells. It has been demonstrated that the αGalCer analogue, threitol ceramide (ThrCer 2), successfully activates iNKT cells and overcomes the problematic iNKT cell activation-induced anergy. In this study, ThrCer 2 has been inserted into the bilayers of liposomes composed of a neutral lipid, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), or dimethyldioctadecylammonium bromide (DDA), a cationic lipid. Incorporation efficiencies of ThrCer within the liposomes was 96% for DSPC liposomes and 80% for DDA liposomes, with the vesicle size (large multilamellar vs. small unilamellar vesicles) making no significant difference. Langmuir-Blodgett studies suggest that both DSPC and DDA stack within the monolayer co-operatively with the ThrCer molecules with no condensing effect. In terms of cellular responses, IFN-γ secretion was higher for cells treated with small DDA liposomes compared with the other liposome formulations, suggesting that ThrCer encapsulation in this liposome formulation resulted in a higher uptake by DCs. |
| first_indexed | 2024-03-07T01:42:28Z |
| format | Journal article |
| id | oxford-uuid:975920fc-6f02-47a0-aa14-3f5f4a058c7e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:42:28Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:975920fc-6f02-47a0-aa14-3f5f4a058c7e2022-03-26T23:58:47ZPreparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:975920fc-6f02-47a0-aa14-3f5f4a058c7eEnglishSymplectic Elements at Oxford2011Kaur, RChen, JDawoodji, ACerundolo, VGarcia-Diaz, YWojno, JCox, LBesra, GMoghaddam, BPerrie, YDendritic cells (DCs) are able to present glycolipids to invariant natural killer T (iNKT) cells in vivo. Very few compounds have been found to stimulate iNKT cells, and of these, the best characterised is the glycolipid α-galactosylceramide, which stimulates the production of large quantities of interferon-gamma (IFN-γ) and interleukin-4 (IL-4). However, αGalCer leads to overstimulation of iNKT cells. It has been demonstrated that the αGalCer analogue, threitol ceramide (ThrCer 2), successfully activates iNKT cells and overcomes the problematic iNKT cell activation-induced anergy. In this study, ThrCer 2 has been inserted into the bilayers of liposomes composed of a neutral lipid, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), or dimethyldioctadecylammonium bromide (DDA), a cationic lipid. Incorporation efficiencies of ThrCer within the liposomes was 96% for DSPC liposomes and 80% for DDA liposomes, with the vesicle size (large multilamellar vs. small unilamellar vesicles) making no significant difference. Langmuir-Blodgett studies suggest that both DSPC and DDA stack within the monolayer co-operatively with the ThrCer molecules with no condensing effect. In terms of cellular responses, IFN-γ secretion was higher for cells treated with small DDA liposomes compared with the other liposome formulations, suggesting that ThrCer encapsulation in this liposome formulation resulted in a higher uptake by DCs. |
| spellingShingle | Kaur, R Chen, J Dawoodji, A Cerundolo, V Garcia-Diaz, Y Wojno, J Cox, L Besra, G Moghaddam, B Perrie, Y Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. |
| title | Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. |
| title_full | Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. |
| title_fullStr | Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. |
| title_full_unstemmed | Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. |
| title_short | Preparation, characterisation and entrapment of a non-glycosidic threitol ceramide into liposomes for presentation to invariant natural killer T cells. |
| title_sort | preparation characterisation and entrapment of a non glycosidic threitol ceramide into liposomes for presentation to invariant natural killer t cells |
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