Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state.
A-kinase anchor proteins (AKAPs) target protein kinase A (PKA) to different subcellular locations and are thought to play important roles in the cAMP signaling pathway. The aims of this study were to determine whether T cells express AKAPs and, if so, to establish their physiological significance. C...
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| Format: | Journal article |
| Language: | English |
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2002
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| _version_ | 1797083522838560768 |
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| author | Williams, R |
| author_facet | Williams, R |
| author_sort | Williams, R |
| collection | OXFORD |
| description | A-kinase anchor proteins (AKAPs) target protein kinase A (PKA) to different subcellular locations and are thought to play important roles in the cAMP signaling pathway. The aims of this study were to determine whether T cells express AKAPs and, if so, to establish their physiological significance. CD4(+) T cells were found to express eight AKAPs. Disruption of the AKAP-PKA interaction caused high levels of IL-2, IL-4, IL-5, and IFN-gamma production in the absence of stimulation via CD3epsilon and CD28 molecules. Disruption of the AKAP-PKA interaction acted synergistically with suboptimal doses of Ag in boosting proliferative responses of T cells. Finally, disruption of the AKAP-PKA interaction rendered T cells insensitive to cAMP-elevating agents. It was concluded that AKAPs, through their association with PKA, are involved in maintaining T cell homeostasis and in regulating the sensitivity of T cells to incoming cAMP signals. |
| first_indexed | 2024-03-07T01:42:48Z |
| format | Journal article |
| id | oxford-uuid:9770da83-299f-4cf2-bf14-551decf6067e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:42:48Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:9770da83-299f-4cf2-bf14-551decf6067e2022-03-26T23:59:32ZCutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9770da83-299f-4cf2-bf14-551decf6067eEnglishSymplectic Elements at Oxford2002Williams, RA-kinase anchor proteins (AKAPs) target protein kinase A (PKA) to different subcellular locations and are thought to play important roles in the cAMP signaling pathway. The aims of this study were to determine whether T cells express AKAPs and, if so, to establish their physiological significance. CD4(+) T cells were found to express eight AKAPs. Disruption of the AKAP-PKA interaction caused high levels of IL-2, IL-4, IL-5, and IFN-gamma production in the absence of stimulation via CD3epsilon and CD28 molecules. Disruption of the AKAP-PKA interaction acted synergistically with suboptimal doses of Ag in boosting proliferative responses of T cells. Finally, disruption of the AKAP-PKA interaction rendered T cells insensitive to cAMP-elevating agents. It was concluded that AKAPs, through their association with PKA, are involved in maintaining T cell homeostasis and in regulating the sensitivity of T cells to incoming cAMP signals. |
| spellingShingle | Williams, R Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state. |
| title | Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state. |
| title_full | Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state. |
| title_fullStr | Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state. |
| title_full_unstemmed | Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state. |
| title_short | Cutting edge: A-kinase anchor proteins are involved in maintaining resting T cells in an inactive state. |
| title_sort | cutting edge a kinase anchor proteins are involved in maintaining resting t cells in an inactive state |
| work_keys_str_mv | AT williamsr cuttingedgeakinaseanchorproteinsareinvolvedinmaintainingrestingtcellsinaninactivestate |