A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border
Clinically useful diagnostic tests of dengue virus infection are lacking. We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undiffer...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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| _version_ | 1797083568520822784 |
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| author | Watthanaworawit, W Turner, P Turner, C Tanganuchitcharnchai, A Jarman, R Blacksell, S Nosten, F |
| author_facet | Watthanaworawit, W Turner, P Turner, C Tanganuchitcharnchai, A Jarman, R Blacksell, S Nosten, F |
| author_sort | Watthanaworawit, W |
| collection | OXFORD |
| description | Clinically useful diagnostic tests of dengue virus infection are lacking. We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. NS-1 antigen and IgM antibody were not sufficiently sensitive to be used as a single confirmatory test with sensitivities of 54% and 17% respectively. The specificities of rRT-PCR, NS-1 antigen and IgM antibody tests were 96%, 100% and 88% respectively. Combining NS-1 and rRT-PCR or the combination of all three tests resulted in the highest sensitivity (93%) but specificities dropped to 96% and 83% respectively. We conclude that at least the combination of two tests, either agent detection (rRT-PCR) or antigen detection (NS-1) plus IgM antibody detection should be used for laboratory confirmation of dengue infection. © 2010 Royal Society of Tropical Medicine and Hygiene. |
| first_indexed | 2024-03-07T01:43:19Z |
| format | Journal article |
| id | oxford-uuid:97909e05-b69a-4595-a9a0-bbf2fbd37078 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:43:19Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:97909e05-b69a-4595-a9a0-bbf2fbd370782022-03-27T00:00:44ZA prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar borderJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:97909e05-b69a-4595-a9a0-bbf2fbd37078EnglishSymplectic Elements at Oxford2011Watthanaworawit, WTurner, PTurner, CTanganuchitcharnchai, AJarman, RBlacksell, SNosten, FClinically useful diagnostic tests of dengue virus infection are lacking. We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. NS-1 antigen and IgM antibody were not sufficiently sensitive to be used as a single confirmatory test with sensitivities of 54% and 17% respectively. The specificities of rRT-PCR, NS-1 antigen and IgM antibody tests were 96%, 100% and 88% respectively. Combining NS-1 and rRT-PCR or the combination of all three tests resulted in the highest sensitivity (93%) but specificities dropped to 96% and 83% respectively. We conclude that at least the combination of two tests, either agent detection (rRT-PCR) or antigen detection (NS-1) plus IgM antibody detection should be used for laboratory confirmation of dengue infection. © 2010 Royal Society of Tropical Medicine and Hygiene. |
| spellingShingle | Watthanaworawit, W Turner, P Turner, C Tanganuchitcharnchai, A Jarman, R Blacksell, S Nosten, F A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border |
| title | A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border |
| title_full | A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border |
| title_fullStr | A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border |
| title_full_unstemmed | A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border |
| title_short | A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border |
| title_sort | prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the thailand myanmar border |
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