Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter.
A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, acc...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1993
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| _version_ | 1797083674836992000 |
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| author | Fodor, E Seong, B Brownlee, G |
| author_facet | Fodor, E Seong, B Brownlee, G |
| author_sort | Fodor, E |
| collection | OXFORD |
| description | A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding. |
| first_indexed | 2024-03-07T01:44:47Z |
| format | Journal article |
| id | oxford-uuid:9809f71a-0e8f-485f-abd6-bd324783c5b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:44:47Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | oxford-uuid:9809f71a-0e8f-485f-abd6-bd324783c5b42022-03-27T00:04:13ZPhotochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9809f71a-0e8f-485f-abd6-bd324783c5b4EnglishSymplectic Elements at Oxford1993Fodor, ESeong, BBrownlee, GA previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding. |
| spellingShingle | Fodor, E Seong, B Brownlee, G Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. |
| title | Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. |
| title_full | Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. |
| title_fullStr | Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. |
| title_full_unstemmed | Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. |
| title_short | Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. |
| title_sort | photochemical cross linking of influenza a polymerase to its virion rna promoter defines a polymerase binding site at residues 9 to 12 of the promoter |
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