The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product.
The mouse cell FT210 was isolated as a G2 phase mutant with a possible defect in the histone H1 kinase. We determined that a temperature-sensitive lesion in this cell line lies in the CDC2 gene. DNA sequence analysis revealed two point mutations in highly conserved regions of the gene: an isoleucine...
| Hoofdauteurs: | , , , , , , |
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
1990
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| _version_ | 1826286516620492800 |
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| author | Th'ng, J Wright, P Hamaguchi, J Lee, MG Norbury, C Nurse, P Bradbury, E |
| author_facet | Th'ng, J Wright, P Hamaguchi, J Lee, MG Norbury, C Nurse, P Bradbury, E |
| author_sort | Th'ng, J |
| collection | OXFORD |
| description | The mouse cell FT210 was isolated as a G2 phase mutant with a possible defect in the histone H1 kinase. We determined that a temperature-sensitive lesion in this cell line lies in the CDC2 gene. DNA sequence analysis revealed two point mutations in highly conserved regions of the gene: an isoleucine to valine change in the PSTAIR region, and a proline to serine change at the C-terminal region of the protein p34. These mutations cause the p34 protein kinase to become inactivated and degraded in FT210 cells at the restrictive temperature, 39 degrees C. The consequence of this temperature-induced inactivation of the CDC2 gene product is cell cycle arrest at the mid to late G2 phase, and this arrest can be alleviated by the introduction of the human CDC2 homolog. |
| first_indexed | 2024-03-07T01:44:56Z |
| format | Journal article |
| id | oxford-uuid:98189768-2d26-4757-bccb-10e030bcd8f2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:44:56Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:98189768-2d26-4757-bccb-10e030bcd8f22022-03-27T00:04:31ZThe FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:98189768-2d26-4757-bccb-10e030bcd8f2EnglishSymplectic Elements at Oxford1990Th'ng, JWright, PHamaguchi, JLee, MGNorbury, CNurse, PBradbury, EThe mouse cell FT210 was isolated as a G2 phase mutant with a possible defect in the histone H1 kinase. We determined that a temperature-sensitive lesion in this cell line lies in the CDC2 gene. DNA sequence analysis revealed two point mutations in highly conserved regions of the gene: an isoleucine to valine change in the PSTAIR region, and a proline to serine change at the C-terminal region of the protein p34. These mutations cause the p34 protein kinase to become inactivated and degraded in FT210 cells at the restrictive temperature, 39 degrees C. The consequence of this temperature-induced inactivation of the CDC2 gene product is cell cycle arrest at the mid to late G2 phase, and this arrest can be alleviated by the introduction of the human CDC2 homolog. |
| spellingShingle | Th'ng, J Wright, P Hamaguchi, J Lee, MG Norbury, C Nurse, P Bradbury, E The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. |
| title | The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. |
| title_full | The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. |
| title_fullStr | The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. |
| title_full_unstemmed | The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. |
| title_short | The FT210 cell line is a mouse G2 phase mutant with a temperature-sensitive CDC2 gene product. |
| title_sort | ft210 cell line is a mouse g2 phase mutant with a temperature sensitive cdc2 gene product |
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