The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA.
The human snRNA genes transcribed by RNA polymerase II (e.g. U1 and U2) have a characteristic TATA-less promoter containing an essential proximal sequence element. Formation of the 3' end of these non-polyadenylated RNAs requires a specialized 3' box element whose function is promoter spec...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1826286678859317248 |
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| author | Medlin, J Uguen, P Taylor, A Bentley, D Murphy, S |
| author_facet | Medlin, J Uguen, P Taylor, A Bentley, D Murphy, S |
| author_sort | Medlin, J |
| collection | OXFORD |
| description | The human snRNA genes transcribed by RNA polymerase II (e.g. U1 and U2) have a characteristic TATA-less promoter containing an essential proximal sequence element. Formation of the 3' end of these non-polyadenylated RNAs requires a specialized 3' box element whose function is promoter specific. Here we show that truncation of the C-terminal domain (CTD) of RNA polymerase II and treatment of cells with CTD kinase inhibitors, including DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole), causes a dramatic reduction in proper 3' end formation of U2 transcripts. Activation of 3' box recognition by the phosphorylated CTD would be consistent with the role of phospho-CTD in mRNA processing. CTD kinase inhibitors, however, have little effect on initiation or elongation of transcription of the U2 genes, whereas elongation of transcription of the beta-actin gene is severely affected. This result highlights differences in transcription of snRNA and mRNA genes. |
| first_indexed | 2024-03-07T01:47:19Z |
| format | Journal article |
| id | oxford-uuid:98de6318-0a4f-4548-8b84-30213aa0753e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:47:19Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:98de6318-0a4f-4548-8b84-30213aa0753e2022-03-27T00:10:02ZThe C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:98de6318-0a4f-4548-8b84-30213aa0753eEnglishSymplectic Elements at Oxford2003Medlin, JUguen, PTaylor, ABentley, DMurphy, SThe human snRNA genes transcribed by RNA polymerase II (e.g. U1 and U2) have a characteristic TATA-less promoter containing an essential proximal sequence element. Formation of the 3' end of these non-polyadenylated RNAs requires a specialized 3' box element whose function is promoter specific. Here we show that truncation of the C-terminal domain (CTD) of RNA polymerase II and treatment of cells with CTD kinase inhibitors, including DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole), causes a dramatic reduction in proper 3' end formation of U2 transcripts. Activation of 3' box recognition by the phosphorylated CTD would be consistent with the role of phospho-CTD in mRNA processing. CTD kinase inhibitors, however, have little effect on initiation or elongation of transcription of the U2 genes, whereas elongation of transcription of the beta-actin gene is severely affected. This result highlights differences in transcription of snRNA and mRNA genes. |
| spellingShingle | Medlin, J Uguen, P Taylor, A Bentley, D Murphy, S The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA. |
| title | The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA. |
| title_full | The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA. |
| title_fullStr | The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA. |
| title_full_unstemmed | The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA. |
| title_short | The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA. |
| title_sort | c terminal domain of pol ii and a drb sensitive kinase are required for 3 processing of u2 snrna |
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