Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.

Crystals of bacteriophage PRD1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection. It has been found that the addition of polyethylene glycol 20...

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Автори: Cockburn, J, Bamford, J, Grimes, J, Bamford, D, Stuart, D
Формат: Journal article
Мова:English
Опубліковано: 2003
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author Cockburn, J
Bamford, J
Grimes, J
Bamford, D
Stuart, D
author_facet Cockburn, J
Bamford, J
Grimes, J
Bamford, D
Stuart, D
author_sort Cockburn, J
collection OXFORD
description Crystals of bacteriophage PRD1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection. It has been found that the addition of polyethylene glycol 20 000 (PEG 20K) to the mother liquor that bathes the crystals allows far higher resolution diffraction intensities to be observed. Growing and treating the crystals in this way has produced a small number of crystals which are particularly amenable to X-ray diffraction analysis.
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spelling oxford-uuid:98f63d07-343a-4fcb-8a99-dc7a4ddcd7ae2022-03-27T00:10:47ZCrystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:98f63d07-343a-4fcb-8a99-dc7a4ddcd7aeEnglishSymplectic Elements at Oxford2003Cockburn, JBamford, JGrimes, JBamford, DStuart, DCrystals of bacteriophage PRD1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection. It has been found that the addition of polyethylene glycol 20 000 (PEG 20K) to the mother liquor that bathes the crystals allows far higher resolution diffraction intensities to be observed. Growing and treating the crystals in this way has produced a small number of crystals which are particularly amenable to X-ray diffraction analysis.
spellingShingle Cockburn, J
Bamford, J
Grimes, J
Bamford, D
Stuart, D
Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.
title Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.
title_full Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.
title_fullStr Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.
title_full_unstemmed Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.
title_short Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.
title_sort crystallization of the membrane containing bacteriophage prd1 in quartz capillaries by vapour diffusion
work_keys_str_mv AT cockburnj crystallizationofthemembranecontainingbacteriophageprd1inquartzcapillariesbyvapourdiffusion
AT bamfordj crystallizationofthemembranecontainingbacteriophageprd1inquartzcapillariesbyvapourdiffusion
AT grimesj crystallizationofthemembranecontainingbacteriophageprd1inquartzcapillariesbyvapourdiffusion
AT bamfordd crystallizationofthemembranecontainingbacteriophageprd1inquartzcapillariesbyvapourdiffusion
AT stuartd crystallizationofthemembranecontainingbacteriophageprd1inquartzcapillariesbyvapourdiffusion