Requirements for T lymphocyte migration in explanted lymph nodes.
Although the requirements for T lymphocyte homing to lymph nodes (LNs) are well studied, much less is known about the requirements for T lymphocyte locomotion within LNs. Imaging of murine T lymphocyte migration in explanted LNs using two-photon laser-scanning fluorescence microscopy provides an opp...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2007
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| _version_ | 1797084102868860928 |
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| author | Huang, J Cárdenas-Navia, L Caldwell, C Plumb, T Radu, C Rocha, P Wilder, T Bromberg, J Cronstein, B Sitkovsky, M Dewhirst, M Dustin, M |
| author_facet | Huang, J Cárdenas-Navia, L Caldwell, C Plumb, T Radu, C Rocha, P Wilder, T Bromberg, J Cronstein, B Sitkovsky, M Dewhirst, M Dustin, M |
| author_sort | Huang, J |
| collection | OXFORD |
| description | Although the requirements for T lymphocyte homing to lymph nodes (LNs) are well studied, much less is known about the requirements for T lymphocyte locomotion within LNs. Imaging of murine T lymphocyte migration in explanted LNs using two-photon laser-scanning fluorescence microscopy provides an opportunity to systematically study these requirements. We have developed a closed system for imaging an intact LN with controlled temperature, oxygenation, and perfusion rate. Naive T lymphocyte locomotion in the deep paracortex of the LN required a perfusion rate of >13 microm/s and a partial pressure of O(2) (pO(2)) of >7.4%. Naive T lymphocyte locomotion in the subcapsular region was 38% slower and had higher turning angles and arrest coefficients than naive T lymphocytes in the deep paracortex. T lymphocyte activation decreased the requirement for pO(2), but also decreased the speed of locomotion in the deep paracortex. Although CCR7(-/-) naive T cells displayed a small reduction in locomotion, systemic treatment with pertussis toxin reduced naive T lymphocyte speed by 59%, indicating a contribution of Galpha(i)-mediated signaling, but involvement of other G protein-coupled receptors besides CCR7. Receptor knockouts or pharmacological inhibition in the adenosine, PG/lipoxygenase, lysophosphatidylcholine, and sphingosine-1-phosphate pathways did not individually alter naive T cell migration. These data implicate pO(2), tissue architecture, and G-protein coupled receptor signaling in regulation of naive T lymphocyte migration in explanted LNs. |
| first_indexed | 2024-03-07T01:50:43Z |
| format | Journal article |
| id | oxford-uuid:99ffa518-4935-4be3-9d4b-99f26c9f4f25 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:50:43Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:99ffa518-4935-4be3-9d4b-99f26c9f4f252022-03-27T00:18:19ZRequirements for T lymphocyte migration in explanted lymph nodes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:99ffa518-4935-4be3-9d4b-99f26c9f4f25EnglishSymplectic Elements at Oxford2007Huang, JCárdenas-Navia, LCaldwell, CPlumb, TRadu, CRocha, PWilder, TBromberg, JCronstein, BSitkovsky, MDewhirst, MDustin, MAlthough the requirements for T lymphocyte homing to lymph nodes (LNs) are well studied, much less is known about the requirements for T lymphocyte locomotion within LNs. Imaging of murine T lymphocyte migration in explanted LNs using two-photon laser-scanning fluorescence microscopy provides an opportunity to systematically study these requirements. We have developed a closed system for imaging an intact LN with controlled temperature, oxygenation, and perfusion rate. Naive T lymphocyte locomotion in the deep paracortex of the LN required a perfusion rate of >13 microm/s and a partial pressure of O(2) (pO(2)) of >7.4%. Naive T lymphocyte locomotion in the subcapsular region was 38% slower and had higher turning angles and arrest coefficients than naive T lymphocytes in the deep paracortex. T lymphocyte activation decreased the requirement for pO(2), but also decreased the speed of locomotion in the deep paracortex. Although CCR7(-/-) naive T cells displayed a small reduction in locomotion, systemic treatment with pertussis toxin reduced naive T lymphocyte speed by 59%, indicating a contribution of Galpha(i)-mediated signaling, but involvement of other G protein-coupled receptors besides CCR7. Receptor knockouts or pharmacological inhibition in the adenosine, PG/lipoxygenase, lysophosphatidylcholine, and sphingosine-1-phosphate pathways did not individually alter naive T cell migration. These data implicate pO(2), tissue architecture, and G-protein coupled receptor signaling in regulation of naive T lymphocyte migration in explanted LNs. |
| spellingShingle | Huang, J Cárdenas-Navia, L Caldwell, C Plumb, T Radu, C Rocha, P Wilder, T Bromberg, J Cronstein, B Sitkovsky, M Dewhirst, M Dustin, M Requirements for T lymphocyte migration in explanted lymph nodes. |
| title | Requirements for T lymphocyte migration in explanted lymph nodes. |
| title_full | Requirements for T lymphocyte migration in explanted lymph nodes. |
| title_fullStr | Requirements for T lymphocyte migration in explanted lymph nodes. |
| title_full_unstemmed | Requirements for T lymphocyte migration in explanted lymph nodes. |
| title_short | Requirements for T lymphocyte migration in explanted lymph nodes. |
| title_sort | requirements for t lymphocyte migration in explanted lymph nodes |
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