Lamin B1 controls oxidative stress responses via Oct-1
Interactions of lamins with chromatin and transcription factors regulate transcription. Oct-1 has previously been shown to colocalize partly with B-type lamins and is essential for transcriptional regulation of oxidative stress response genes. Using sequential extraction, co-immunoprecipitation (IP)...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Rockefeller University Press
2009
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| Subjects: |
| _version_ | 1797084103064944640 |
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| author | Malhas, A Lee, C Vaux, D |
| author_facet | Malhas, A Lee, C Vaux, D |
| author_sort | Malhas, A |
| collection | OXFORD |
| description | Interactions of lamins with chromatin and transcription factors regulate transcription. Oct-1 has previously been shown to colocalize partly with B-type lamins and is essential for transcriptional regulation of oxidative stress response genes. Using sequential extraction, co-immunoprecipitation (IP), fluorescence loss in photobleaching, and fluorescence resonance energy transfer, we confirm Oct-1-lamin B1 association at the nuclear periphery and show that this association is lost in Lmnb1<sup>Δ/Δ</sup> cells. We show that several Oct-1-dependent genes, including a subset involved in oxidative stress response, are dysregulated in mnb1<sup>Δ/Δ</sup> cells. Electrophoretic mobility shift assay and chromatin IP reveal that Oct-1 binds to the putative octamer-binding sequences of the dysregulated genes and that this activity is increased in cells lacking functional lamin B1. Like Oct1 <sup>-/-</sup> cells, Lmnb1<sup>Δ/Δ</sup> cells have elevated levels of reactive oxygen species and are more susceptible to oxidative stress. Sequestration of Oct-1 at the nuclear periphery by lamin B1 may be a mechanism by which the nuclear envelope can regulate gene expression and contribute to the cellular response to stress, development, and aging. |
| first_indexed | 2024-03-07T01:50:43Z |
| format | Journal article |
| id | oxford-uuid:99ffbcee-71cb-45fc-acff-2e664eda935d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:50:43Z |
| publishDate | 2009 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | oxford-uuid:99ffbcee-71cb-45fc-acff-2e664eda935d2022-03-27T00:18:17ZLamin B1 controls oxidative stress responses via Oct-1Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:99ffbcee-71cb-45fc-acff-2e664eda935dPhysicsPathologyEnglishOxford University Research Archive - ValetRockefeller University Press2009Malhas, ALee, CVaux, DInteractions of lamins with chromatin and transcription factors regulate transcription. Oct-1 has previously been shown to colocalize partly with B-type lamins and is essential for transcriptional regulation of oxidative stress response genes. Using sequential extraction, co-immunoprecipitation (IP), fluorescence loss in photobleaching, and fluorescence resonance energy transfer, we confirm Oct-1-lamin B1 association at the nuclear periphery and show that this association is lost in Lmnb1<sup>Δ/Δ</sup> cells. We show that several Oct-1-dependent genes, including a subset involved in oxidative stress response, are dysregulated in mnb1<sup>Δ/Δ</sup> cells. Electrophoretic mobility shift assay and chromatin IP reveal that Oct-1 binds to the putative octamer-binding sequences of the dysregulated genes and that this activity is increased in cells lacking functional lamin B1. Like Oct1 <sup>-/-</sup> cells, Lmnb1<sup>Δ/Δ</sup> cells have elevated levels of reactive oxygen species and are more susceptible to oxidative stress. Sequestration of Oct-1 at the nuclear periphery by lamin B1 may be a mechanism by which the nuclear envelope can regulate gene expression and contribute to the cellular response to stress, development, and aging. |
| spellingShingle | Physics Pathology Malhas, A Lee, C Vaux, D Lamin B1 controls oxidative stress responses via Oct-1 |
| title | Lamin B1 controls oxidative stress responses via Oct-1 |
| title_full | Lamin B1 controls oxidative stress responses via Oct-1 |
| title_fullStr | Lamin B1 controls oxidative stress responses via Oct-1 |
| title_full_unstemmed | Lamin B1 controls oxidative stress responses via Oct-1 |
| title_short | Lamin B1 controls oxidative stress responses via Oct-1 |
| title_sort | lamin b1 controls oxidative stress responses via oct 1 |
| topic | Physics Pathology |
| work_keys_str_mv | AT malhasa laminb1controlsoxidativestressresponsesviaoct1 AT leec laminb1controlsoxidativestressresponsesviaoct1 AT vauxd laminb1controlsoxidativestressresponsesviaoct1 |