Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease
Heterogeneity of lymphatic vessels during embryogenesis is critical for organ-specific lymphatic function. Little is known about lymphatics in the developing kidney, despite their established roles in pathology of the mature organ. We performed three-dimensional imaging to characterize lymphatic ves...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
eLife Sciences Publications
2019
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| _version_ | 1797084183098556416 |
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| author | Jafree, D Moulding, D Kolatsi-Joannou, M Perretta Tejedor, N Price, K Milmoe, N Walsh, C Correra, R Winyard, P Harris, P Ruhrberg, C Walker-Samuel, S Riley, P Woolf, A Scambler, P Long, D |
| author_facet | Jafree, D Moulding, D Kolatsi-Joannou, M Perretta Tejedor, N Price, K Milmoe, N Walsh, C Correra, R Winyard, P Harris, P Ruhrberg, C Walker-Samuel, S Riley, P Woolf, A Scambler, P Long, D |
| author_sort | Jafree, D |
| collection | OXFORD |
| description | Heterogeneity of lymphatic vessels during embryogenesis is critical for organ-specific lymphatic function. Little is known about lymphatics in the developing kidney, despite their established roles in pathology of the mature organ. We performed three-dimensional imaging to characterize lymphatic vessel formation in the mammalian embryonic kidney at single-cell resolution. In mouse, we visually and quantitatively assessed the development of kidney lymphatic vessels, remodeling from a ring-like anastomosis under the nascent renal pelvis, a site of VEGF-C expression, to form a patent vascular plexus. We identified a heterogenous population of lymphatic endothelial cell clusters in mouse and human embryonic kidneys. Exogenous VEGF-C expanded the lymphatic population in explanted mouse embryonic kidneys. Finally, we characterized complex kidney lymphatic abnormalities in a genetic mouse model of polycystic kidney disease. Our study provides novel insights into the development of kidney lymphatic vasculature; a system which likely has fundamental roles in renal development, physiology and disease. |
| first_indexed | 2024-03-07T01:51:48Z |
| format | Journal article |
| id | oxford-uuid:9a586a63-ea5c-462a-8c6f-730d21b2f2af |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:51:48Z |
| publishDate | 2019 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:9a586a63-ea5c-462a-8c6f-730d21b2f2af2022-03-27T00:20:48ZSpatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney diseaseJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9a586a63-ea5c-462a-8c6f-730d21b2f2afEnglishSymplectic Elements at OxfordeLife Sciences Publications2019Jafree, DMoulding, DKolatsi-Joannou, MPerretta Tejedor, NPrice, KMilmoe, NWalsh, CCorrera, RWinyard, PHarris, PRuhrberg, CWalker-Samuel, SRiley, PWoolf, AScambler, PLong, DHeterogeneity of lymphatic vessels during embryogenesis is critical for organ-specific lymphatic function. Little is known about lymphatics in the developing kidney, despite their established roles in pathology of the mature organ. We performed three-dimensional imaging to characterize lymphatic vessel formation in the mammalian embryonic kidney at single-cell resolution. In mouse, we visually and quantitatively assessed the development of kidney lymphatic vessels, remodeling from a ring-like anastomosis under the nascent renal pelvis, a site of VEGF-C expression, to form a patent vascular plexus. We identified a heterogenous population of lymphatic endothelial cell clusters in mouse and human embryonic kidneys. Exogenous VEGF-C expanded the lymphatic population in explanted mouse embryonic kidneys. Finally, we characterized complex kidney lymphatic abnormalities in a genetic mouse model of polycystic kidney disease. Our study provides novel insights into the development of kidney lymphatic vasculature; a system which likely has fundamental roles in renal development, physiology and disease. |
| spellingShingle | Jafree, D Moulding, D Kolatsi-Joannou, M Perretta Tejedor, N Price, K Milmoe, N Walsh, C Correra, R Winyard, P Harris, P Ruhrberg, C Walker-Samuel, S Riley, P Woolf, A Scambler, P Long, D Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
| title | Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
| title_full | Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
| title_fullStr | Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
| title_full_unstemmed | Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
| title_short | Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
| title_sort | spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease |
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