Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR
<p><strong>Background:</strong><br /> Some COVID-19 cases test positive again for SARS-CoV-2 RNA following negative test results and discharge, raising questions about the meaning of virus detection. Better characterization of re-positive cases is urgently needed.</p>&l...
| Asıl Yazarlar: | , , , , , , , , , , , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Elsevier
2020
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| _version_ | 1826287009276100608 |
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| author | Lu, J Peng, J Xiong, Q Liu, Z Lin, H Tan, X Kang, M Yuan, R Zeng, L Zhou, P Liang, C Yi, L du Plessis, L Song, T Ma, W Sun, J Pybus, OG Ke, C |
| author_facet | Lu, J Peng, J Xiong, Q Liu, Z Lin, H Tan, X Kang, M Yuan, R Zeng, L Zhou, P Liang, C Yi, L du Plessis, L Song, T Ma, W Sun, J Pybus, OG Ke, C |
| author_sort | Lu, J |
| collection | OXFORD |
| description | <p><strong>Background:</strong><br />
Some COVID-19 cases test positive again for SARS-CoV-2 RNA following negative test results and discharge, raising questions about the meaning of virus detection. Better characterization of re-positive cases is urgently needed.</p><br />
<p><strong>Methods:</strong><br />
Clinical data were obtained through Guangdong's COVID-19 surveillance network. Neutralization antibody titre was determined using microneutralization assays. Potential infectivity of clinical samples was evaluated by cell inoculation. SARS-CoV-2 RNA was detected using three different RT-PCR kits and multiplex PCR with nanopore sequencing.</p><br />
<p><strong>Findings:</strong><br />
Among 619 discharged COVID-19 cases, 87 re-tested as SARS-CoV-2 positive in circumstances of social isolation. All re-positive cases had mild or moderate symptoms at initial diagnosis and were younger on average (median, 28). Re-positive cases (n = 59) exhibited similar neutralization antibodies (NAbs) titre distributions to other COVID-19 cases (n = 218) tested here. No infectious strain could be obtained by culture and no full-length viral genomes could be sequenced from re-positive cases.</p><br />
<p><strong>Interpretation:</strong><br />
Re-positive SARS-CoV-2 cases do not appear to be caused by active reinfection and were identified in ~14% of discharged cases. A robust NAb response and potential virus genome degradation were detected in almost all re-positive cases, suggesting a substantially lower transmission risk, especially through respiratory routes.</p> |
| first_indexed | 2024-03-07T01:52:11Z |
| format | Journal article |
| id | oxford-uuid:9a7ef8b2-65f4-427e-b34d-864df7fbe5c7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:52:11Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:9a7ef8b2-65f4-427e-b34d-864df7fbe5c72022-03-27T00:21:43ZClinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCRJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9a7ef8b2-65f4-427e-b34d-864df7fbe5c7EnglishSymplectic ElementsElsevier2020Lu, JPeng, JXiong, QLiu, ZLin, HTan, XKang, MYuan, RZeng, LZhou, PLiang, CYi, Ldu Plessis, LSong, TMa, WSun, JPybus, OGKe, C<p><strong>Background:</strong><br /> Some COVID-19 cases test positive again for SARS-CoV-2 RNA following negative test results and discharge, raising questions about the meaning of virus detection. Better characterization of re-positive cases is urgently needed.</p><br /> <p><strong>Methods:</strong><br /> Clinical data were obtained through Guangdong's COVID-19 surveillance network. Neutralization antibody titre was determined using microneutralization assays. Potential infectivity of clinical samples was evaluated by cell inoculation. SARS-CoV-2 RNA was detected using three different RT-PCR kits and multiplex PCR with nanopore sequencing.</p><br /> <p><strong>Findings:</strong><br /> Among 619 discharged COVID-19 cases, 87 re-tested as SARS-CoV-2 positive in circumstances of social isolation. All re-positive cases had mild or moderate symptoms at initial diagnosis and were younger on average (median, 28). Re-positive cases (n = 59) exhibited similar neutralization antibodies (NAbs) titre distributions to other COVID-19 cases (n = 218) tested here. No infectious strain could be obtained by culture and no full-length viral genomes could be sequenced from re-positive cases.</p><br /> <p><strong>Interpretation:</strong><br /> Re-positive SARS-CoV-2 cases do not appear to be caused by active reinfection and were identified in ~14% of discharged cases. A robust NAb response and potential virus genome degradation were detected in almost all re-positive cases, suggesting a substantially lower transmission risk, especially through respiratory routes.</p> |
| spellingShingle | Lu, J Peng, J Xiong, Q Liu, Z Lin, H Tan, X Kang, M Yuan, R Zeng, L Zhou, P Liang, C Yi, L du Plessis, L Song, T Ma, W Sun, J Pybus, OG Ke, C Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR |
| title | Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR |
| title_full | Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR |
| title_fullStr | Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR |
| title_full_unstemmed | Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR |
| title_short | Clinical, immunological and virological characterization of COVID-19 patients that test re-positive for SARS-CoV-2 by RT-PCR |
| title_sort | clinical immunological and virological characterization of covid 19 patients that test re positive for sars cov 2 by rt pcr |
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