Isozyme expression and gene-centromere distances in diploid and triploid hybrid catfish
Allozyme expression of 14 loci was studied in diploid channel catfish Ictalurus punctatus, blue catfish I. furcatus, and diploid and triploid hybrid catfish (channel catfish X blue catfish). This allowed appropriate interpretation of allozyme expression and estimates of gene-centromere distances in...
| Główni autorzy: | , , , , |
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| Format: | Journal article |
| Język: | English |
| Wydane: |
1996
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| Streszczenie: | Allozyme expression of 14 loci was studied in diploid channel catfish Ictalurus punctatus, blue catfish I. furcatus, and diploid and triploid hybrid catfish (channel catfish X blue catfish). This allowed appropriate interpretation of allozyme expression and estimates of gene-centromere distances in triploid hybrid catfish and contributed to genetic characterization of the commercially important ictalurid catfishes. Five polymorphic loci in the parental female channel catfish were used to confirm the retention of an extra diagnostic maternal allele in triploid offspring. Fidelity of gene dosage and allozyme expression of three parental alleles were found at 13 of 14 loci of triploid progeny; however, expression of phosphoglucomutase (PGM) in 174 and 197 triploid hybrids was similar to that of diploid hybrids. Electrophoretic variation for PGM was observed when different buffers were used, indicating nongenetic alteration of expression. Two polymorphic loci in triploid hybrid catfish provided new gene-centromere distances of 14 centimorgans (cM) for aspartate aminotransferase (mAAT*) and 45 cM for glucose-6-phosphate isomerase (GPI-A*). No differences were found in recombination rates of isocitrate dehydrogenase (IDHP*) and peptidase-5 (PEPS-1*) loci of triploid hybrids at different ages. © Copyright by the American Fisheries Society 1996. |
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