The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide.
Activation of the oxidized inactive state (termed Unready or Ni(u)) of the [NiFe]-hydrogenase from Allochromatium vinosum requires removal of an unidentified oxidizing entity [O], produced by partial reduction of O(2). Dynamic electrochemical kinetic studies, subjecting enzyme molecules on an electr...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2005
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| _version_ | 1826287289012060160 |
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| author | Lamle, SE Albracht, S Armstrong, F |
| author_facet | Lamle, SE Albracht, S Armstrong, F |
| author_sort | Lamle, SE |
| collection | OXFORD |
| description | Activation of the oxidized inactive state (termed Unready or Ni(u)) of the [NiFe]-hydrogenase from Allochromatium vinosum requires removal of an unidentified oxidizing entity [O], produced by partial reduction of O(2). Dynamic electrochemical kinetic studies, subjecting enzyme molecules on an electrode to sequences of potential steps and gas injections, establish the order of events in an otherwise complex sequence of reactions that involves more than one intermediate retaining [O] or its redox equivalent; fast and reversible electron transfer precedes the rate-determining step which is followed by a reaction with H(2), or the inhibitor CO, that renders the reductive activation process irreversible. |
| first_indexed | 2024-03-07T01:56:25Z |
| format | Journal article |
| id | oxford-uuid:9bdbf633-74e9-44cf-a601-13d8a664657d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T01:56:25Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:9bdbf633-74e9-44cf-a601-13d8a664657d2022-03-27T00:31:53ZThe mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9bdbf633-74e9-44cf-a601-13d8a664657dEnglishSymplectic Elements at Oxford2005Lamle, SEAlbracht, SArmstrong, FActivation of the oxidized inactive state (termed Unready or Ni(u)) of the [NiFe]-hydrogenase from Allochromatium vinosum requires removal of an unidentified oxidizing entity [O], produced by partial reduction of O(2). Dynamic electrochemical kinetic studies, subjecting enzyme molecules on an electrode to sequences of potential steps and gas injections, establish the order of events in an otherwise complex sequence of reactions that involves more than one intermediate retaining [O] or its redox equivalent; fast and reversible electron transfer precedes the rate-determining step which is followed by a reaction with H(2), or the inhibitor CO, that renders the reductive activation process irreversible. |
| spellingShingle | Lamle, SE Albracht, S Armstrong, F The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide. |
| title | The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide. |
| title_full | The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide. |
| title_fullStr | The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide. |
| title_full_unstemmed | The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide. |
| title_short | The mechanism of activation of a [NiFe]-hydrogenase by electrons, hydrogen, and carbon monoxide. |
| title_sort | mechanism of activation of a nife hydrogenase by electrons hydrogen and carbon monoxide |
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