Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam
Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled f...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Centers for Disease Control and Prevention
2020
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| _version_ | 1826287530529521664 |
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| author | Anh, NT Nhu, LNT Hong, NTT Phuc, TM Tam, PTT Huong, DT Anh, TT Deng, X Nghia, HDT Nguyen, TT Van Hung, N Thuan, ND Phuong, PTH Chau, NVV Baker, S Delwart, E Thwaites, G Van Tan, L VIZIONS Consortium1 |
| author_facet | Anh, NT Nhu, LNT Hong, NTT Phuc, TM Tam, PTT Huong, DT Anh, TT Deng, X Nghia, HDT Nguyen, TT Van Hung, N Thuan, ND Phuong, PTH Chau, NVV Baker, S Delwart, E Thwaites, G Van Tan, L VIZIONS Consortium1 |
| author_sort | Anh, NT |
| collection | OXFORD |
| description | Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012–2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections. |
| first_indexed | 2024-03-07T02:00:04Z |
| format | Journal article |
| id | oxford-uuid:9d0ed3de-5248-4a94-ab8a-c793f63d0bce |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:00:04Z |
| publishDate | 2020 |
| publisher | Centers for Disease Control and Prevention |
| record_format | dspace |
| spelling | oxford-uuid:9d0ed3de-5248-4a94-ab8a-c793f63d0bce2022-03-27T00:40:23ZViral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, VietnamJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9d0ed3de-5248-4a94-ab8a-c793f63d0bceEnglishSymplectic ElementsCenters for Disease Control and Prevention2020Anh, NTNhu, LNTHong, NTTPhuc, TMTam, PTTHuong, DTAnh, TTDeng, XNghia, HDTNguyen, TTVan Hung, NThuan, NDPhuong, PTHChau, NVVBaker, SDelwart, EThwaites, GVan Tan, LVIZIONS Consortium1Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012–2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections. |
| spellingShingle | Anh, NT Nhu, LNT Hong, NTT Phuc, TM Tam, PTT Huong, DT Anh, TT Deng, X Nghia, HDT Nguyen, TT Van Hung, N Thuan, ND Phuong, PTH Chau, NVV Baker, S Delwart, E Thwaites, G Van Tan, L VIZIONS Consortium1 Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| title | Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| title_full | Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| title_fullStr | Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| title_full_unstemmed | Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| title_short | Viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin, Vietnam |
| title_sort | viral metagenomic analysis of cerebrospinal fluid from patients with acute central nervous system infections of unknown origin vietnam |
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