Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide
In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which all...
| Главные авторы: | , , , , , , , |
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| Формат: | Journal article |
| Язык: | English |
| Опубликовано: |
American Chemical Society
2015
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| _version_ | 1826287701472575488 |
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| author | Alonso-Cristobal, P Vilela, P El-Sagheer, A Lopez-Cabarcos, E Brown, T Muskens, O Rubio-Retama, J Kanaras, A |
| author_facet | Alonso-Cristobal, P Vilela, P El-Sagheer, A Lopez-Cabarcos, E Brown, T Muskens, O Rubio-Retama, J Kanaras, A |
| author_sort | Alonso-Cristobal, P |
| collection | OXFORD |
| description | In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the π-π stacking interaction between the nucleobases of the DNA and the sp(2) carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM. |
| first_indexed | 2024-03-07T02:02:36Z |
| format | Journal article |
| id | oxford-uuid:9ddf98fb-b75e-4884-8d5e-753c7c7e1e5a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:02:36Z |
| publishDate | 2015 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:9ddf98fb-b75e-4884-8d5e-753c7c7e1e5a2022-03-27T00:46:18ZHighly sensitive DNA sensor based on upconversion nanoparticles and graphene oxideJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9ddf98fb-b75e-4884-8d5e-753c7c7e1e5aEnglishSymplectic Elements at OxfordAmerican Chemical Society2015Alonso-Cristobal, PVilela, PEl-Sagheer, ALopez-Cabarcos, EBrown, TMuskens, ORubio-Retama, JKanaras, AIn this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 ± 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the π-π stacking interaction between the nucleobases of the DNA and the sp(2) carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM. |
| spellingShingle | Alonso-Cristobal, P Vilela, P El-Sagheer, A Lopez-Cabarcos, E Brown, T Muskens, O Rubio-Retama, J Kanaras, A Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide |
| title | Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide |
| title_full | Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide |
| title_fullStr | Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide |
| title_full_unstemmed | Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide |
| title_short | Highly sensitive DNA sensor based on upconversion nanoparticles and graphene oxide |
| title_sort | highly sensitive dna sensor based on upconversion nanoparticles and graphene oxide |
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