Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens.
Alpha-toxin of Clostridium perfringens, cloned in Escherichia coli, has been purified and crystallized from ammonium sulphate using the hanging drop vapour diffusion method at 20 degrees C. The crystals diffract to a minimum Bragg spacing of 2.7 A, belong to the space group R32 (with a = b = 153.3 A...
| Autori principali: | , , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
1994
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| _version_ | 1826287736515985408 |
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| author | Basak, A Stuart, D Nikura, T Bishop, D Kelly, D Fearn, A Titball, R |
| author_facet | Basak, A Stuart, D Nikura, T Bishop, D Kelly, D Fearn, A Titball, R |
| author_sort | Basak, A |
| collection | OXFORD |
| description | Alpha-toxin of Clostridium perfringens, cloned in Escherichia coli, has been purified and crystallized from ammonium sulphate using the hanging drop vapour diffusion method at 20 degrees C. The crystals diffract to a minimum Bragg spacing of 2.7 A, belong to the space group R32 (with a = b = 153.3 A, c = 95.4 A, alpha = beta = 90 degrees and gamma = 120 degrees) and contain a single polypeptide chain in the crystallographic unit. |
| first_indexed | 2024-03-07T02:03:07Z |
| format | Journal article |
| id | oxford-uuid:9e0cea2f-e8ec-409f-ab0a-8bf7b5112c1e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:03:07Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:9e0cea2f-e8ec-409f-ab0a-8bf7b5112c1e2022-03-27T00:47:21ZPurification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9e0cea2f-e8ec-409f-ab0a-8bf7b5112c1eEnglishSymplectic Elements at Oxford1994Basak, AStuart, DNikura, TBishop, DKelly, DFearn, ATitball, RAlpha-toxin of Clostridium perfringens, cloned in Escherichia coli, has been purified and crystallized from ammonium sulphate using the hanging drop vapour diffusion method at 20 degrees C. The crystals diffract to a minimum Bragg spacing of 2.7 A, belong to the space group R32 (with a = b = 153.3 A, c = 95.4 A, alpha = beta = 90 degrees and gamma = 120 degrees) and contain a single polypeptide chain in the crystallographic unit. |
| spellingShingle | Basak, A Stuart, D Nikura, T Bishop, D Kelly, D Fearn, A Titball, R Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens. |
| title | Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens. |
| title_full | Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens. |
| title_fullStr | Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens. |
| title_full_unstemmed | Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens. |
| title_short | Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens. |
| title_sort | purification crystallization and preliminary x ray diffraction studies of alpha toxin of clostridium perfringens |
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