Novel display of knotted DNA molecules by two-dimensional gel electrophoresis.
We describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate according to their compactness. The second gel dimension is run at high voltage, and DNA knots migrate accor...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2001
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| _version_ | 1797085016017076224 |
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| author | Trigueros, S Arsuaga, J Vazquez, M Sumners, D Roca, J |
| author_facet | Trigueros, S Arsuaga, J Vazquez, M Sumners, D Roca, J |
| author_sort | Trigueros, S |
| collection | OXFORD |
| description | We describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate according to their compactness. The second gel dimension is run at high voltage, and DNA knots migrate according to other physical parameters such as shape and flexibility. In comparison with one-dimensional gel electrophoresis, this procedure segregates the knotted DNA molecules from other unknotted forms of DNA, and partially resolves populations of knots that have the same number of crossings. The two-dimensional display may allow quantitative and qualitative characterization of different types of DNA knots simply by gel velocity. |
| first_indexed | 2024-03-07T02:03:15Z |
| format | Journal article |
| id | oxford-uuid:9e1a90c4-8ac4-442f-bf5a-0dff7d2e9135 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:03:15Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:9e1a90c4-8ac4-442f-bf5a-0dff7d2e91352022-03-27T00:47:45ZNovel display of knotted DNA molecules by two-dimensional gel electrophoresis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9e1a90c4-8ac4-442f-bf5a-0dff7d2e9135EnglishSymplectic Elements at Oxford2001Trigueros, SArsuaga, JVazquez, MSumners, DRoca, JWe describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate according to their compactness. The second gel dimension is run at high voltage, and DNA knots migrate according to other physical parameters such as shape and flexibility. In comparison with one-dimensional gel electrophoresis, this procedure segregates the knotted DNA molecules from other unknotted forms of DNA, and partially resolves populations of knots that have the same number of crossings. The two-dimensional display may allow quantitative and qualitative characterization of different types of DNA knots simply by gel velocity. |
| spellingShingle | Trigueros, S Arsuaga, J Vazquez, M Sumners, D Roca, J Novel display of knotted DNA molecules by two-dimensional gel electrophoresis. |
| title | Novel display of knotted DNA molecules by two-dimensional gel electrophoresis. |
| title_full | Novel display of knotted DNA molecules by two-dimensional gel electrophoresis. |
| title_fullStr | Novel display of knotted DNA molecules by two-dimensional gel electrophoresis. |
| title_full_unstemmed | Novel display of knotted DNA molecules by two-dimensional gel electrophoresis. |
| title_short | Novel display of knotted DNA molecules by two-dimensional gel electrophoresis. |
| title_sort | novel display of knotted dna molecules by two dimensional gel electrophoresis |
| work_keys_str_mv | AT trigueross noveldisplayofknotteddnamoleculesbytwodimensionalgelelectrophoresis AT arsuagaj noveldisplayofknotteddnamoleculesbytwodimensionalgelelectrophoresis AT vazquezm noveldisplayofknotteddnamoleculesbytwodimensionalgelelectrophoresis AT sumnersd noveldisplayofknotteddnamoleculesbytwodimensionalgelelectrophoresis AT rocaj noveldisplayofknotteddnamoleculesbytwodimensionalgelelectrophoresis |