Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry.
Mass spectrometry (MS) is a recognized approach for characterizing proteins and the complexes they assemble into. This application of a long-established physico-chemical tool to the frontiers of structural biology has stemmed from experiments performed in the early 1990s. While initial studies focus...
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1826287832134582272 |
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| author | Hilton, G Benesch, J |
| author_facet | Hilton, G Benesch, J |
| author_sort | Hilton, G |
| collection | OXFORD |
| description | Mass spectrometry (MS) is a recognized approach for characterizing proteins and the complexes they assemble into. This application of a long-established physico-chemical tool to the frontiers of structural biology has stemmed from experiments performed in the early 1990s. While initial studies focused on the elucidation of stoichiometry by means of simple mass determination, developments in MS technology and methodology now allow researchers to address questions of shape, inter-subunit connectivity and protein dynamics. Here, we chart the remarkable rise of MS and its application to biomolecular complexes over the last two decades. |
| first_indexed | 2024-03-07T02:04:34Z |
| format | Journal article |
| id | oxford-uuid:9e84a891-a9df-4afe-9108-dc311d4d549f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:04:34Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:9e84a891-a9df-4afe-9108-dc311d4d549f2022-03-27T00:50:39ZTwo decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9e84a891-a9df-4afe-9108-dc311d4d549fEnglishSymplectic Elements at Oxford2012Hilton, GBenesch, JMass spectrometry (MS) is a recognized approach for characterizing proteins and the complexes they assemble into. This application of a long-established physico-chemical tool to the frontiers of structural biology has stemmed from experiments performed in the early 1990s. While initial studies focused on the elucidation of stoichiometry by means of simple mass determination, developments in MS technology and methodology now allow researchers to address questions of shape, inter-subunit connectivity and protein dynamics. Here, we chart the remarkable rise of MS and its application to biomolecular complexes over the last two decades. |
| spellingShingle | Hilton, G Benesch, J Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry. |
| title | Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry. |
| title_full | Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry. |
| title_fullStr | Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry. |
| title_full_unstemmed | Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry. |
| title_short | Two decades of studying non-covalent biomolecular assemblies by means of electrospray ionization mass spectrometry. |
| title_sort | two decades of studying non covalent biomolecular assemblies by means of electrospray ionization mass spectrometry |
| work_keys_str_mv | AT hiltong twodecadesofstudyingnoncovalentbiomolecularassembliesbymeansofelectrosprayionizationmassspectrometry AT beneschj twodecadesofstudyingnoncovalentbiomolecularassembliesbymeansofelectrosprayionizationmassspectrometry |