Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins.
Fluorescent proteins have had a great impact on the way in which plant membrane traffic is studied. Here we review the uses to which these molecules have been put in this field of research and discuss the advantages and pitfalls of particular fluorescent protein derivatives in various applications a...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1797085165819789312 |
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| author | Samalova, M Fricker, M Moore, I |
| author_facet | Samalova, M Fricker, M Moore, I |
| author_sort | Samalova, M |
| collection | OXFORD |
| description | Fluorescent proteins have had a great impact on the way in which plant membrane traffic is studied. Here we review the uses to which these molecules have been put in this field of research and discuss the advantages and pitfalls of particular fluorescent protein derivatives in various applications and plant species. We discuss in detail the need for quantitative estimates of expression level and the potential of fluorescent proteins for quantitative assays of biosynthetic membrane traffic. Detailed descriptions and protocols are provided for the use of the newly developed 2A-based ratiometric polyprotein probes of membrane traffic in conjunction with semiautomated image analysis software packages for quantitative analyses. The ratiometric probes and software are available from the authors. |
| first_indexed | 2024-03-07T02:05:06Z |
| format | Journal article |
| id | oxford-uuid:9eb7b899-9863-4b8e-afdd-55e52c262653 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:05:06Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:9eb7b899-9863-4b8e-afdd-55e52c2626532022-03-27T00:52:09ZQuantitative and qualitative analysis of plant membrane traffic using fluorescent proteins.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9eb7b899-9863-4b8e-afdd-55e52c262653EnglishSymplectic Elements at Oxford2008Samalova, MFricker, MMoore, IFluorescent proteins have had a great impact on the way in which plant membrane traffic is studied. Here we review the uses to which these molecules have been put in this field of research and discuss the advantages and pitfalls of particular fluorescent protein derivatives in various applications and plant species. We discuss in detail the need for quantitative estimates of expression level and the potential of fluorescent proteins for quantitative assays of biosynthetic membrane traffic. Detailed descriptions and protocols are provided for the use of the newly developed 2A-based ratiometric polyprotein probes of membrane traffic in conjunction with semiautomated image analysis software packages for quantitative analyses. The ratiometric probes and software are available from the authors. |
| spellingShingle | Samalova, M Fricker, M Moore, I Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. |
| title | Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. |
| title_full | Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. |
| title_fullStr | Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. |
| title_full_unstemmed | Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. |
| title_short | Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. |
| title_sort | quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins |
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