Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.

Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.

Studying the structure and dynamics of proteins in live cells is essential to understanding their physiological activities and mechanisms, and to validating in vitro characterization. Improvements in labeling and imaging technologies are starting to allow such in vivo studies; however, a number of t...

Descrizione completa

Dettagli Bibliografici
Autori principali:	Sustarsic, M, Plochowietz, A, Aigrain, L, Yuzenkova, Y, Zenkin, N, Kapanidis, A
Natura:	Journal article
Lingua:	English
Pubblicazione:	2014

Documenti analoghi

Optimized Internalization of Fluorescently Labeled Biomolecules into Live Bacteria
di: Sustarsic, M, et al.
Pubblicazione: (2014)

Single-Molecule Fluorescence and FRET Measurements on Internalized Proteins in Living Bacteria
di: Aigrain, L, et al.
Pubblicazione: (2013)

Long-lived intracellular single-molecule fluorescence using electroporated molecules.
di: Crawford, R, et al.
Pubblicazione: (2013)

Internalization of Fluorescent Biomolecules for Long-Lived Single-Molecule Observation in Living Bacteria
di: Crawford, R, et al.
Pubblicazione: (2013)

Characterization of organic fluorophores for in vivo FRET studies based on electroporated molecules.
di: Plochowietz, A, et al.
Pubblicazione: (2014)

Single-molecule fluorescence of internalised biomolecules in live bacteria
di: Plochowietz, A
Pubblicazione: (2012)

Imaging of single dye-labeled chemotaxis proteins in live bacteria using electroporation
di: Di Paolo, D, et al.
Pubblicazione: (2018)

In vivo single-RNA tracking shows that most tRNA diffuses freely in live bacteria
di: Plochowietz, A, et al.
Pubblicazione: (2016)

Single-molecule imaging of electroporated chemotaxis proteins in live bacteria
di: Di Paolo, D
Pubblicazione: (2015)

Single-molecule FRET studies in live bacteria
di: Plochowietz, A
Pubblicazione: (2016)

Stable end-sealed DNA as robust nano-rulers for in vivo single-molecule fluorescence
di: Plochowietz, A, et al.
Pubblicazione: (2016)

Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates.
di: Kapanidis, A, et al.
Pubblicazione: (2001)

Single-molecule FRET measurements in bacterial cells
di: Aigrain, L, et al.
Pubblicazione: (2012)

Stable transformation of fluorescent proteins into Nosema bombycis by electroporation
di: Zhanqi Dong, et al.
Pubblicazione: (2022-04-01)

Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
di: Sascha Conic, et al.
Pubblicazione: (2018-11-01)

Aberrant topologies of bacterial membrane proteins revealed by high sensitivity fluorescence labelling
di: Hickman, SJ, et al.
Pubblicazione: (2023)

Confinement-free wide-field ratiometric tracking of single fluorescent molecules
di: Gilboa, B, et al.
Pubblicazione: (2019)

Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase
di: David Forrest, et al.
Pubblicazione: (2017-06-01)

Single-molecule imaging of electroporated dye-labelled CheY in live E. coli
di: Berry, R, et al.
Pubblicazione: (2016)

Single-molecule imaging of electroporated dye-labelled CheY in live Escherichia coli
di: Di Paolo, D, et al.
Pubblicazione: (2016)

Single-Molecule Imaging of Electroporated Dye-Labelled CheY in Live E. coli
di: Berry, R, et al.
Pubblicazione: (2016)

Single-Molecule FRET in Living Bacteria
di: Crawford, R, et al.
Pubblicazione: (2012)

Single-molecule and super-resolution imaging of transcription in living bacteria
di: Stracy, M, et al.
Pubblicazione: (2017)

Reversible Electroporation–Mediated Liposomal Doxorubicin Delivery to Tumors Can Be Monitored With Zr-Labeled Reporter Nanoparticles
di: Govindarajan Srimathveeravalli PhD, et al.
Pubblicazione: (2018-02-01)

Minimal genetically encoded tags for fluorescent protein labeling in living neurons
di: Aleksandra Arsić, et al.
Pubblicazione: (2022-01-01)

Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFv
di: Ioannis Tsirkas, et al.
Pubblicazione: (2023-06-01)

Rapid single-cell electroporation for labeling organotypic cultures
di: Steinmeyer, Joseph D. (Joseph Daly)
Pubblicazione: (2010)

Electroporation of tissue and cells for drug delivery applications
di: Prausnitz, Mark R
Pubblicazione: (2006)

A Review on Electroporation-Based Intracellular Delivery
di: Junfeng Shi, et al.
Pubblicazione: (2018-11-01)

Well Plate–Based Localized Electroporation Workflow for Rapid Optimization of Intracellular Delivery
di: Cesar Patino, et al.
Pubblicazione: (2024-07-01)

Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins
di: Hannah E. Chia, et al.
Pubblicazione: (2020-09-01)

Choosing the right label for single-molecule tracking in live bacteria: side-by-side comparison of photoactivatable fluorescent protein and Halo tag dyes
di: Banaz, N, et al.
Pubblicazione: (2018)

Transfer of Green Fluorescent Protein (GFP) Gene to Betta splendens Embryos by Transfection and Electroporation Methods
di: Eni Kusrini, et al.
Pubblicazione: (2018-08-01)

Improved tracking and resolution of bacteria in holographic microscopy using dye and fluorescent protein labeling
di: Jay eNadeau, et al.
Pubblicazione: (2016-04-01)

Labeling Proteins Inside Living Cells Using External Fluorophores for Fluorescence Microscopy
di: Kai Wen Teng, et al.
Pubblicazione: (2017-01-01)

Evaluation and Optimization of Protein Extraction From E. coli by Electroporation
di: Saša Haberl Meglič, et al.
Pubblicazione: (2020-09-01)

Inactivation of antibiotic-resistant bacteria Escherichia coli by electroporation
di: Saša Haberl Meglič, et al.
Pubblicazione: (2024-01-01)

Fluorescently Labelled Tau Protein
di: Saurabh Awasthi, et al.
Pubblicazione: (2023-12-01)

In vivo electroporation mediated gene delivery to the beating heart.
di: Erick L Ayuni, et al.
Pubblicazione: (2010-01-01)

Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase
di: Craggs, T, et al.
Pubblicazione: (2019)