Characterising electrosprayed biomolecules using tandem-MS - the noncovalent GroEL chaperonin assembly

Characterising electrosprayed biomolecules using tandem-MS - the noncovalent GroEL chaperonin assembly

It is now possible to transfer large protein complexes intact into the gas phase using nano-electrospray ionisation (ESI) and to investigate their stoichiometry in a mass spectrometer. Using as a model assembly the noncovalent 14mer (ca. 800 kDa) of the chaperonin GroEL from Escherichia coli we show...

Bibliografske podrobnosti
Main Authors:	Sobott, F, Robinson, C
Format:	Journal article
Jezik:	English
Izdano:	2004

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