Characterising electrosprayed biomolecules using tandem-MS - the noncovalent GroEL chaperonin assembly

It is now possible to transfer large protein complexes intact into the gas phase using nano-electrospray ionisation (ESI) and to investigate their stoichiometry in a mass spectrometer. Using as a model assembly the noncovalent 14mer (ca. 800 kDa) of the chaperonin GroEL from Escherichia coli we show...

Disgrifiad llawn

Manylion Llyfryddiaeth
Prif Awduron: Sobott, F, Robinson, C
Fformat: Journal article
Iaith:English
Cyhoeddwyd: 2004