Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains
3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a populat...
| Main Authors: | , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
MPDI Open Access Journals
2019
|
| _version_ | 1797085376894992384 |
|---|---|
| author | Oldach, P Nieduszynski, C |
| author_facet | Oldach, P Nieduszynski, C |
| author_sort | Oldach, P |
| collection | OXFORD |
| description | 3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1. View Full-Text. |
| first_indexed | 2024-03-07T02:08:07Z |
| format | Journal article |
| id | oxford-uuid:9facaa86-a5be-407f-a542-a734a5ff4ece |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:08:07Z |
| publishDate | 2019 |
| publisher | MPDI Open Access Journals |
| record_format | dspace |
| spelling | oxford-uuid:9facaa86-a5be-407f-a542-a734a5ff4ece2022-03-27T00:59:45ZCohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing DomainsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9facaa86-a5be-407f-a542-a734a5ff4eceEnglishSymplectic Elements at OxfordMPDI Open Access Journals2019Oldach, PNieduszynski, C3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1. View Full-Text. |
| spellingShingle | Oldach, P Nieduszynski, C Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains |
| title | Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains |
| title_full | Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains |
| title_fullStr | Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains |
| title_full_unstemmed | Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains |
| title_short | Cohesin-Mediated Genome Architecture Does Not Define DNA Replication Timing Domains |
| title_sort | cohesin mediated genome architecture does not define dna replication timing domains |
| work_keys_str_mv | AT oldachp cohesinmediatedgenomearchitecturedoesnotdefinednareplicationtimingdomains AT nieduszynskic cohesinmediatedgenomearchitecturedoesnotdefinednareplicationtimingdomains |