Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis.
Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisi...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2013
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| _version_ | 1826288073329082368 |
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| author | Eichinger, C Kurze, A Oliveira, R Nasmyth, K |
| author_facet | Eichinger, C Kurze, A Oliveira, R Nasmyth, K |
| author_sort | Eichinger, C |
| collection | OXFORD |
| description | Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct. |
| first_indexed | 2024-03-07T02:08:13Z |
| format | Journal article |
| id | oxford-uuid:9fb680ee-95ec-4337-805d-8f798c276f4f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:08:13Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:9fb680ee-95ec-4337-805d-8f798c276f4f2022-03-27T00:59:56ZDisengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9fb680ee-95ec-4337-805d-8f798c276f4fEnglishSymplectic Elements at Oxford2013Eichinger, CKurze, AOliveira, RNasmyth, KCohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct. |
| spellingShingle | Eichinger, C Kurze, A Oliveira, R Nasmyth, K Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. |
| title | Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. |
| title_full | Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. |
| title_fullStr | Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. |
| title_full_unstemmed | Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. |
| title_short | Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. |
| title_sort | disengaging the smc3 kleisin interface releases cohesin from drosophila chromosomes during interphase and mitosis |
| work_keys_str_mv | AT eichingerc disengagingthesmc3kleisininterfacereleasescohesinfromdrosophilachromosomesduringinterphaseandmitosis AT kurzea disengagingthesmc3kleisininterfacereleasescohesinfromdrosophilachromosomesduringinterphaseandmitosis AT oliveirar disengagingthesmc3kleisininterfacereleasescohesinfromdrosophilachromosomesduringinterphaseandmitosis AT nasmythk disengagingthesmc3kleisininterfacereleasescohesinfromdrosophilachromosomesduringinterphaseandmitosis |