Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease
Determining the etiological basis of central nervous system (CNS) infections is inherently challenging, primarily due to the multi-etiological nature. Using RNA sequencing, we aimed to identify microbes present in cerebrospinal fluid (CSF) of two patients suffering CNS infection, previously diagnose...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2019
|
| _version_ | 1826288133549850624 |
|---|---|
| author | Pettersson, J Piorkowski, G Mayxay, M Rattanavong, S Vongsouvath, M Davong, V Alfsnes, K Eldholm, V De Lamballerie, X Holmes, E Newton, P Dubot-Pérès, A |
| author_facet | Pettersson, J Piorkowski, G Mayxay, M Rattanavong, S Vongsouvath, M Davong, V Alfsnes, K Eldholm, V De Lamballerie, X Holmes, E Newton, P Dubot-Pérès, A |
| author_sort | Pettersson, J |
| collection | OXFORD |
| description | Determining the etiological basis of central nervous system (CNS) infections is inherently challenging, primarily due to the multi-etiological nature. Using RNA sequencing, we aimed to identify microbes present in cerebrospinal fluid (CSF) of two patients suffering CNS infection, previously diagnosed with Cryptococcus sp. and Streptococcus pneumoniae infection, respectively. After meta-transcriptomic analysis, and confirmation with real-time PCR, hepatitis B virus (HBV) was detected in the CSF of two patients diagnosed with CNS syndrome. Phylogenetic analysis of the partial HBV genomes from these patients showed that they belonged to genotypes B and C and clustered with other viruses of Asian origin. In countries with high levels of HBV endemicity, the virus is likely to be found in patients diagnosed with CNS infections, although whether it contributes to symptoms and pathology, or is simply a coincidental infection, is unknown and merits further investigation. |
| first_indexed | 2024-03-07T02:09:07Z |
| format | Journal article |
| id | oxford-uuid:9ffeb744-a430-4757-bce2-5c885c38682f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:09:07Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:9ffeb744-a430-4757-bce2-5c885c38682f2022-03-27T02:02:11ZMeta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system diseaseJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:9ffeb744-a430-4757-bce2-5c885c38682fEnglishSymplectic Elements at OxfordElsevier2019Pettersson, JPiorkowski, GMayxay, MRattanavong, SVongsouvath, MDavong, VAlfsnes, KEldholm, VDe Lamballerie, XHolmes, ENewton, PDubot-Pérès, ADetermining the etiological basis of central nervous system (CNS) infections is inherently challenging, primarily due to the multi-etiological nature. Using RNA sequencing, we aimed to identify microbes present in cerebrospinal fluid (CSF) of two patients suffering CNS infection, previously diagnosed with Cryptococcus sp. and Streptococcus pneumoniae infection, respectively. After meta-transcriptomic analysis, and confirmation with real-time PCR, hepatitis B virus (HBV) was detected in the CSF of two patients diagnosed with CNS syndrome. Phylogenetic analysis of the partial HBV genomes from these patients showed that they belonged to genotypes B and C and clustered with other viruses of Asian origin. In countries with high levels of HBV endemicity, the virus is likely to be found in patients diagnosed with CNS infections, although whether it contributes to symptoms and pathology, or is simply a coincidental infection, is unknown and merits further investigation. |
| spellingShingle | Pettersson, J Piorkowski, G Mayxay, M Rattanavong, S Vongsouvath, M Davong, V Alfsnes, K Eldholm, V De Lamballerie, X Holmes, E Newton, P Dubot-Pérès, A Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease |
| title | Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease |
| title_full | Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease |
| title_fullStr | Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease |
| title_full_unstemmed | Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease |
| title_short | Meta-transcriptomic identification of hepatitis B virus in cerebrospinal fluid in patients with central nervous system disease |
| title_sort | meta transcriptomic identification of hepatitis b virus in cerebrospinal fluid in patients with central nervous system disease |
| work_keys_str_mv | AT petterssonj metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT piorkowskig metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT mayxaym metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT rattanavongs metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT vongsouvathm metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT davongv metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT alfsnesk metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT eldholmv metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT delamballeriex metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT holmese metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT newtonp metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease AT dubotperesa metatranscriptomicidentificationofhepatitisbvirusincerebrospinalfluidinpatientswithcentralnervoussystemdisease |