The role of skin-homing T cells in extrinsic atopic dermatitis.
BACKGROUND: T cells that express Cutaneous Lymphocyte-Associated antigen (CLA) have the potential of migrating to the skin, and are hypothesized to play a role in cutaneous atopic disease. AIM: To investigate the immune phenotype and cytokine responses to Der p 1 stimulation of CLA+ T cells in extri...
| Main Authors: | , , , , |
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| פורמט: | Journal article |
| שפה: | English |
| יצא לאור: |
2007
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| _version_ | 1826288202929930240 |
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| author | Seneviratne, S Black, A Jones, L Bailey, A Ogg, G |
| author_facet | Seneviratne, S Black, A Jones, L Bailey, A Ogg, G |
| author_sort | Seneviratne, S |
| collection | OXFORD |
| description | BACKGROUND: T cells that express Cutaneous Lymphocyte-Associated antigen (CLA) have the potential of migrating to the skin, and are hypothesized to play a role in cutaneous atopic disease. AIM: To investigate the immune phenotype and cytokine responses to Der p 1 stimulation of CLA+ T cells in extrinsic atopic dermatitis (EAD). DESIGN: In vitro testing, with controls. METHODS: Peripheral blood mononuclear cells (PBMC) were obtained from EAD patients (n=27) and non-atopic healthy individuals (n=22). Phenotypic analysis of naive, CLA+ and non-CLA+ memory/effector CD4+ and CD8+ T cells used markers of cell activation, differentiation, adhesion, apoptosis and chemokine receptor expression. Cytokine responses in these cells were studied following Der p 1 stimulation. RESULTS: CLA+ T cells from EAD patients expressed significantly higher levels of CD25, HLA-DR, CD38, CD71, CXCR1, CXCR2 and lower levels of bcl2, CCR5, CCR7, CXCR3, and CD62L (p<0.05). DISCUSSION: In EAD patients, CLA+ T cells express increased levels of markers associated with activation, adhesion and apoptosis, show differences in the level of expression of differentiation markers and display a distinct chemokine receptor preference, compared with cells from healthy controls. These data suggest a significant role for CLA+ T cells in the pathogenesis of cutaneous atopic disease. |
| first_indexed | 2024-03-07T02:10:09Z |
| format | Journal article |
| id | oxford-uuid:a05a75f3-b00a-4acf-b15c-248b5b1854b4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:10:09Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:a05a75f3-b00a-4acf-b15c-248b5b1854b42022-03-27T02:04:51ZThe role of skin-homing T cells in extrinsic atopic dermatitis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a05a75f3-b00a-4acf-b15c-248b5b1854b4EnglishSymplectic Elements at Oxford2007Seneviratne, SBlack, AJones, LBailey, AOgg, GBACKGROUND: T cells that express Cutaneous Lymphocyte-Associated antigen (CLA) have the potential of migrating to the skin, and are hypothesized to play a role in cutaneous atopic disease. AIM: To investigate the immune phenotype and cytokine responses to Der p 1 stimulation of CLA+ T cells in extrinsic atopic dermatitis (EAD). DESIGN: In vitro testing, with controls. METHODS: Peripheral blood mononuclear cells (PBMC) were obtained from EAD patients (n=27) and non-atopic healthy individuals (n=22). Phenotypic analysis of naive, CLA+ and non-CLA+ memory/effector CD4+ and CD8+ T cells used markers of cell activation, differentiation, adhesion, apoptosis and chemokine receptor expression. Cytokine responses in these cells were studied following Der p 1 stimulation. RESULTS: CLA+ T cells from EAD patients expressed significantly higher levels of CD25, HLA-DR, CD38, CD71, CXCR1, CXCR2 and lower levels of bcl2, CCR5, CCR7, CXCR3, and CD62L (p<0.05). DISCUSSION: In EAD patients, CLA+ T cells express increased levels of markers associated with activation, adhesion and apoptosis, show differences in the level of expression of differentiation markers and display a distinct chemokine receptor preference, compared with cells from healthy controls. These data suggest a significant role for CLA+ T cells in the pathogenesis of cutaneous atopic disease. |
| spellingShingle | Seneviratne, S Black, A Jones, L Bailey, A Ogg, G The role of skin-homing T cells in extrinsic atopic dermatitis. |
| title | The role of skin-homing T cells in extrinsic atopic dermatitis. |
| title_full | The role of skin-homing T cells in extrinsic atopic dermatitis. |
| title_fullStr | The role of skin-homing T cells in extrinsic atopic dermatitis. |
| title_full_unstemmed | The role of skin-homing T cells in extrinsic atopic dermatitis. |
| title_short | The role of skin-homing T cells in extrinsic atopic dermatitis. |
| title_sort | role of skin homing t cells in extrinsic atopic dermatitis |
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