Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice
The microbiota confers colonization resistance, which blocks Salmonella gut colonization1. As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmon...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Nature Research
2019
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| _version_ | 1797085640935866368 |
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| author | Wotzka, S Kreuzer, M Maier, L Arnoldini, M Nguyen, B Brachmann, A Berthold, D Zünd, M Hausmann, A Bakkeren, E Hoces, D Gül, E Beutler, M Dolowschiak, T Zimmermann, M Fuhrer, T Moor, K Sauer, U Typas, A Piel, J Diard, M Macpherson, A Stecher, B Sunagawa, S Slack, E Hardt, W |
| author_facet | Wotzka, S Kreuzer, M Maier, L Arnoldini, M Nguyen, B Brachmann, A Berthold, D Zünd, M Hausmann, A Bakkeren, E Hoces, D Gül, E Beutler, M Dolowschiak, T Zimmermann, M Fuhrer, T Moor, K Sauer, U Typas, A Piel, J Diard, M Macpherson, A Stecher, B Sunagawa, S Slack, E Hardt, W |
| author_sort | Wotzka, S |
| collection | OXFORD |
| description | The microbiota confers colonization resistance, which blocks Salmonella gut colonization1. As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmonella Typhimurium or Escherichia coli gut colonization and plasmid transfer. Here, we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet. Salmonella gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required Salmonella’s AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted Salmonella gut colonization. Both E. coli and Salmonella show much higher bile resistance than the microbiota. Correspondingly, competitive E. coli can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote S. Typhimurium gut infections in mice lacking E. coli in their microbiota. This mouse model may be useful for studying pathogen–microbiota–host interactions, the protective effect of E. coli, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process. |
| first_indexed | 2024-03-07T02:11:21Z |
| format | Journal article |
| id | oxford-uuid:a0c29287-802f-4368-b9fd-50a22f6f8d8d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:11:21Z |
| publishDate | 2019 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:a0c29287-802f-4368-b9fd-50a22f6f8d8d2022-03-27T02:07:56ZEscherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in miceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a0c29287-802f-4368-b9fd-50a22f6f8d8dEnglishSymplectic Elements at OxfordNature Research2019Wotzka, SKreuzer, MMaier, LArnoldini, MNguyen, BBrachmann, ABerthold, DZünd, MHausmann, ABakkeren, EHoces, DGül, EBeutler, MDolowschiak, TZimmermann, MFuhrer, TMoor, KSauer, UTypas, APiel, JDiard, MMacpherson, AStecher, BSunagawa, SSlack, EHardt, WThe microbiota confers colonization resistance, which blocks Salmonella gut colonization1. As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmonella Typhimurium or Escherichia coli gut colonization and plasmid transfer. Here, we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet. Salmonella gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required Salmonella’s AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted Salmonella gut colonization. Both E. coli and Salmonella show much higher bile resistance than the microbiota. Correspondingly, competitive E. coli can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote S. Typhimurium gut infections in mice lacking E. coli in their microbiota. This mouse model may be useful for studying pathogen–microbiota–host interactions, the protective effect of E. coli, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process. |
| spellingShingle | Wotzka, S Kreuzer, M Maier, L Arnoldini, M Nguyen, B Brachmann, A Berthold, D Zünd, M Hausmann, A Bakkeren, E Hoces, D Gül, E Beutler, M Dolowschiak, T Zimmermann, M Fuhrer, T Moor, K Sauer, U Typas, A Piel, J Diard, M Macpherson, A Stecher, B Sunagawa, S Slack, E Hardt, W Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice |
| title | Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice |
| title_full | Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice |
| title_fullStr | Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice |
| title_full_unstemmed | Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice |
| title_short | Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice |
| title_sort | escherichia coli limits salmonella typhimurium infections after diet shifts and fat mediated microbiota perturbation in mice |
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