Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum.
In the mutant BEN210 of Physarum polycephalum several beta-tubulins are detectable. beta 1-tubulin is unique to the myxamoeba, beta 2-tubulin is unique to the plasmodium, and the mutant beta 1-210 tubulin encoded by the benD210 allele is present in both cell types. In order to analyse the subcellula...
| Autors principals: | , , |
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| Format: | Journal article |
| Idioma: | English |
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1989
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| _version_ | 1826288365721354240 |
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| author | Paul, E Burland, T Gull, K |
| author_facet | Paul, E Burland, T Gull, K |
| author_sort | Paul, E |
| collection | OXFORD |
| description | In the mutant BEN210 of Physarum polycephalum several beta-tubulins are detectable. beta 1-tubulin is unique to the myxamoeba, beta 2-tubulin is unique to the plasmodium, and the mutant beta 1-210 tubulin encoded by the benD210 allele is present in both cell types. In order to analyse the subcellular distribution of the beta 1-210 polypeptide, we prepared cytoskeletons from myxamoebae and mitotic spindles from plasmodia, and examined the tubulin polypeptide composition of these microtubular organelles by two-dimensional gel electrophoresis and immunoblotting. The results show that the beta 1-210 tubulin is present in microtubules of both the cytoskeleton and the intranuclear mitotic spindle. Thus a single beta-tubulin gene product can participate in multiple microtubular organelles in distinct cellular compartments. |
| first_indexed | 2024-03-07T02:12:36Z |
| format | Journal article |
| id | oxford-uuid:a128a1db-52dd-4903-b586-713ecbad5f1a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:12:36Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:a128a1db-52dd-4903-b586-713ecbad5f1a2022-03-27T02:10:59ZLocation of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a128a1db-52dd-4903-b586-713ecbad5f1aEnglishSymplectic Elements at Oxford1989Paul, EBurland, TGull, KIn the mutant BEN210 of Physarum polycephalum several beta-tubulins are detectable. beta 1-tubulin is unique to the myxamoeba, beta 2-tubulin is unique to the plasmodium, and the mutant beta 1-210 tubulin encoded by the benD210 allele is present in both cell types. In order to analyse the subcellular distribution of the beta 1-210 polypeptide, we prepared cytoskeletons from myxamoebae and mitotic spindles from plasmodia, and examined the tubulin polypeptide composition of these microtubular organelles by two-dimensional gel electrophoresis and immunoblotting. The results show that the beta 1-210 tubulin is present in microtubules of both the cytoskeleton and the intranuclear mitotic spindle. Thus a single beta-tubulin gene product can participate in multiple microtubular organelles in distinct cellular compartments. |
| spellingShingle | Paul, E Burland, T Gull, K Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum. |
| title | Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum. |
| title_full | Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum. |
| title_fullStr | Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum. |
| title_full_unstemmed | Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum. |
| title_short | Location of a single beta-tubulin gene product in both cytoskeletal and mitotic-spindle microtubules in Physarum polycephalum. |
| title_sort | location of a single beta tubulin gene product in both cytoskeletal and mitotic spindle microtubules in physarum polycephalum |
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