Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa.
The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at lo...
| Glavni autori: | , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
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BioMed Central
2003
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| _version_ | 1826288377014517760 |
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| author | Ochong, E Nzila, A Kimani, S Kokwaro, G Mutabingwa, T Watkins, W Marsh, K |
| author_facet | Ochong, E Nzila, A Kimani, S Kokwaro, G Mutabingwa, T Watkins, W Marsh, K |
| author_sort | Ochong, E |
| collection | OXFORD |
| description | The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation. |
| first_indexed | 2024-03-07T02:12:46Z |
| format | Journal article |
| id | oxford-uuid:a137a56d-d54d-48e9-841f-d0c9a80b2389 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:12:46Z |
| publishDate | 2003 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:a137a56d-d54d-48e9-841f-d0c9a80b23892022-03-27T02:11:23ZMolecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a137a56d-d54d-48e9-841f-d0c9a80b2389EnglishSymplectic Elements at OxfordBioMed Central2003Ochong, ENzila, AKimani, SKokwaro, GMutabingwa, TWatkins, WMarsh, KThe selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998-1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation. |
| spellingShingle | Ochong, E Nzila, A Kimani, S Kokwaro, G Mutabingwa, T Watkins, W Marsh, K Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. |
| title | Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. |
| title_full | Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. |
| title_fullStr | Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. |
| title_full_unstemmed | Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. |
| title_short | Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. |
| title_sort | molecular monitoring of the leu 164 mutation of dihydrofolate reductase in a highly sulfadoxine pyrimethamine resistant area in africa |
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