Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor.
Dynamic and structural information has been obtained for an analogue of acetylcholine while bound to the agonist binding site on the nicotinic acetylcholine receptor (nAcChoR), using wide-line deuterium solid-state NMR. Analysis of the deuterium lineshape obtained at various temperatures from unorie...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2001
|
| _version_ | 1826288458909351936 |
|---|---|
| author | Williamson, P Watts, J Addona, G Miller, K Watts, A |
| author_facet | Williamson, P Watts, J Addona, G Miller, K Watts, A |
| author_sort | Williamson, P |
| collection | OXFORD |
| description | Dynamic and structural information has been obtained for an analogue of acetylcholine while bound to the agonist binding site on the nicotinic acetylcholine receptor (nAcChoR), using wide-line deuterium solid-state NMR. Analysis of the deuterium lineshape obtained at various temperatures from unoriented nAcChoR membranes labeled with deuterated bromoacetylcholine (BAC) showed that the quaternary ammonium group of the ligand is well constrained within the agonist binding site when compared with the dynamics observed in the crystalline solids. This motional restriction would suggest that a high degree of complementarity exists between the quaternary ammonium group of the ligand and the protein within the agonist binding site. nAcChoR membranes were uniaxially oriented by isopotential centrifugation as determined by phosphorous NMR of the membrane phospholipids. Analysis of the deuterium NMR lineshape of these oriented membranes enriched with the nAcChoR labeled with N(+)(CD(3))(3)-BAC has enabled us to determine that the angle formed between the quaternary ammonium group of the BAC and the membrane normal is 42 degrees in the desensitized form of the receptor. This measurement allows us to orient in part the bound ligand within the proposed receptor binding site. |
| first_indexed | 2024-03-07T02:14:00Z |
| format | Journal article |
| id | oxford-uuid:a19c4dcb-2e3d-47c9-b921-a3639dee8074 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:14:00Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:a19c4dcb-2e3d-47c9-b921-a3639dee80742022-03-27T02:14:23ZDynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a19c4dcb-2e3d-47c9-b921-a3639dee8074EnglishSymplectic Elements at Oxford2001Williamson, PWatts, JAddona, GMiller, KWatts, ADynamic and structural information has been obtained for an analogue of acetylcholine while bound to the agonist binding site on the nicotinic acetylcholine receptor (nAcChoR), using wide-line deuterium solid-state NMR. Analysis of the deuterium lineshape obtained at various temperatures from unoriented nAcChoR membranes labeled with deuterated bromoacetylcholine (BAC) showed that the quaternary ammonium group of the ligand is well constrained within the agonist binding site when compared with the dynamics observed in the crystalline solids. This motional restriction would suggest that a high degree of complementarity exists between the quaternary ammonium group of the ligand and the protein within the agonist binding site. nAcChoR membranes were uniaxially oriented by isopotential centrifugation as determined by phosphorous NMR of the membrane phospholipids. Analysis of the deuterium NMR lineshape of these oriented membranes enriched with the nAcChoR labeled with N(+)(CD(3))(3)-BAC has enabled us to determine that the angle formed between the quaternary ammonium group of the BAC and the membrane normal is 42 degrees in the desensitized form of the receptor. This measurement allows us to orient in part the bound ligand within the proposed receptor binding site. |
| spellingShingle | Williamson, P Watts, J Addona, G Miller, K Watts, A Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. |
| title | Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. |
| title_full | Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. |
| title_fullStr | Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. |
| title_full_unstemmed | Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. |
| title_short | Dynamics and orientation of N+(CD3)3-bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor. |
| title_sort | dynamics and orientation of n cd3 3 bromoacetylcholine bound to its binding site on the nicotinic acetylcholine receptor |
| work_keys_str_mv | AT williamsonp dynamicsandorientationofncd33bromoacetylcholineboundtoitsbindingsiteonthenicotinicacetylcholinereceptor AT wattsj dynamicsandorientationofncd33bromoacetylcholineboundtoitsbindingsiteonthenicotinicacetylcholinereceptor AT addonag dynamicsandorientationofncd33bromoacetylcholineboundtoitsbindingsiteonthenicotinicacetylcholinereceptor AT millerk dynamicsandorientationofncd33bromoacetylcholineboundtoitsbindingsiteonthenicotinicacetylcholinereceptor AT wattsa dynamicsandorientationofncd33bromoacetylcholineboundtoitsbindingsiteonthenicotinicacetylcholinereceptor |