Raman activated cell sorting
Single cell Raman spectra (SCRS) are intrinsic biochemical profiles and ‘chemical images’ of single cells which can be used to characterise phenotypic changes, physiological states and functions of cells. On the base of SCRS, Raman activated cell sorting (RACS) provides a label-free cell sorting app...
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| Format: | Journal article |
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Elsevier
2016
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| _version_ | 1797085875232833536 |
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| author | Song, Y Yin, H Huang, W |
| author_facet | Song, Y Yin, H Huang, W |
| author_sort | Song, Y |
| collection | OXFORD |
| description | Single cell Raman spectra (SCRS) are intrinsic biochemical profiles and ‘chemical images’ of single cells which can be used to characterise phenotypic changes, physiological states and functions of cells. On the base of SCRS, Raman activated cell sorting (RACS) provides a label-free cell sorting approach, which can link single cells to their chemical or phenotypic profiles. Overcoming naturally weak Raman signals, establishing Raman biomarker as sorting criteria to RACS and improving specific sorting technology are three challenges of developing RACS. Advances on Raman spectroscopy such as stimulated Raman scattering (SRS) and pre-screening helped to increase RACS sorting speed. Entire SCRS can be characterised using pattern recognition methods, and specific Raman bands can be extracted as biomarkers for RACS. Recent advances on cell sorting technologies based on microfluidic device and surface-ejection enable accurate and reliable single cell sorting from complex samples. A high throughput RACS will be achievable in near future by integrating fast Raman detection system such as SRS with microfluidic RACS and Raman activated cell ejection (RACE). |
| first_indexed | 2024-03-07T02:14:06Z |
| format | Journal article |
| id | oxford-uuid:a1a19b9c-3ad1-48cd-9f47-dad150c18d26 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T02:14:06Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:a1a19b9c-3ad1-48cd-9f47-dad150c18d262022-03-27T02:14:36ZRaman activated cell sortingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a1a19b9c-3ad1-48cd-9f47-dad150c18d26Symplectic Elements at OxfordElsevier2016Song, YYin, HHuang, WSingle cell Raman spectra (SCRS) are intrinsic biochemical profiles and ‘chemical images’ of single cells which can be used to characterise phenotypic changes, physiological states and functions of cells. On the base of SCRS, Raman activated cell sorting (RACS) provides a label-free cell sorting approach, which can link single cells to their chemical or phenotypic profiles. Overcoming naturally weak Raman signals, establishing Raman biomarker as sorting criteria to RACS and improving specific sorting technology are three challenges of developing RACS. Advances on Raman spectroscopy such as stimulated Raman scattering (SRS) and pre-screening helped to increase RACS sorting speed. Entire SCRS can be characterised using pattern recognition methods, and specific Raman bands can be extracted as biomarkers for RACS. Recent advances on cell sorting technologies based on microfluidic device and surface-ejection enable accurate and reliable single cell sorting from complex samples. A high throughput RACS will be achievable in near future by integrating fast Raman detection system such as SRS with microfluidic RACS and Raman activated cell ejection (RACE). |
| spellingShingle | Song, Y Yin, H Huang, W Raman activated cell sorting |
| title | Raman activated cell sorting |
| title_full | Raman activated cell sorting |
| title_fullStr | Raman activated cell sorting |
| title_full_unstemmed | Raman activated cell sorting |
| title_short | Raman activated cell sorting |
| title_sort | raman activated cell sorting |
| work_keys_str_mv | AT songy ramanactivatedcellsorting AT yinh ramanactivatedcellsorting AT huangw ramanactivatedcellsorting |