New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy.
Confocal and multiphoton microscopes are particularly sensitive to specimen- or system-induced aberrations, which result in decreased resolution and signal-to-noise ratio. The inclusion of an adaptive optics correction system could help overcome this limitation and restore diffraction-limited perfor...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2002
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| _version_ | 1797085923749396480 |
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| author | Booth, M Neil, M Wilson, T |
| author_facet | Booth, M Neil, M Wilson, T |
| author_sort | Booth, M |
| collection | OXFORD |
| description | Confocal and multiphoton microscopes are particularly sensitive to specimen- or system-induced aberrations, which result in decreased resolution and signal-to-noise ratio. The inclusion of an adaptive optics correction system could help overcome this limitation and restore diffraction-limited performance, but such a system requires a suitable method of wave-front measurement. By extending the concept of a modal wave-front sensor previously described by Neil et al. [J. Opt. Soc. Am. A 17, 1098-1107 (2000)], we present a new sensor capable of measuring directly the Zernike aberration modes introduced by a specimen. This modal sensor is particularly suited to applications in three-dimensional microscopy because of its inherent axial selectivity; only those wave fronts originating in the focal region contribute to the measured signal. Four wave-front sensor configurations are presented and their input response is characterized. Sensitivity matrices and axial responses are presented. |
| first_indexed | 2024-03-07T02:14:44Z |
| format | Journal article |
| id | oxford-uuid:a1d5c215-3f12-4ed3-8bc1-20f9a8db2336 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:14:44Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:a1d5c215-3f12-4ed3-8bc1-20f9a8db23362022-03-27T02:16:02ZNew modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a1d5c215-3f12-4ed3-8bc1-20f9a8db2336EnglishSymplectic Elements at Oxford2002Booth, MNeil, MWilson, TConfocal and multiphoton microscopes are particularly sensitive to specimen- or system-induced aberrations, which result in decreased resolution and signal-to-noise ratio. The inclusion of an adaptive optics correction system could help overcome this limitation and restore diffraction-limited performance, but such a system requires a suitable method of wave-front measurement. By extending the concept of a modal wave-front sensor previously described by Neil et al. [J. Opt. Soc. Am. A 17, 1098-1107 (2000)], we present a new sensor capable of measuring directly the Zernike aberration modes introduced by a specimen. This modal sensor is particularly suited to applications in three-dimensional microscopy because of its inherent axial selectivity; only those wave fronts originating in the focal region contribute to the measured signal. Four wave-front sensor configurations are presented and their input response is characterized. Sensitivity matrices and axial responses are presented. |
| spellingShingle | Booth, M Neil, M Wilson, T New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. |
| title | New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. |
| title_full | New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. |
| title_fullStr | New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. |
| title_full_unstemmed | New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. |
| title_short | New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. |
| title_sort | new modal wave front sensor application to adaptive confocal fluorescence microscopy and two photon excitation fluorescence microscopy |
| work_keys_str_mv | AT boothm newmodalwavefrontsensorapplicationtoadaptiveconfocalfluorescencemicroscopyandtwophotonexcitationfluorescencemicroscopy AT neilm newmodalwavefrontsensorapplicationtoadaptiveconfocalfluorescencemicroscopyandtwophotonexcitationfluorescencemicroscopy AT wilsont newmodalwavefrontsensorapplicationtoadaptiveconfocalfluorescencemicroscopyandtwophotonexcitationfluorescencemicroscopy |