Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam
Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Published: |
F1000Research
2018
|
| _version_ | 1797085961947971584 |
|---|---|
| author | Thi Ty Hang, V Thi Han Ny, N My Phuc, T Thi Thanh Tam, P Thao Huong, D Dang Trung Nghia, H Tran Anh Vu, N Thi Hong Phuong, P Van Xang, N Dong, N Nhu Hiep, P Van Hung, N Tinh Hien, T Rabaa, M Thwaites, G Baker, S Van Tan, L van Doorn, H |
| author_facet | Thi Ty Hang, V Thi Han Ny, N My Phuc, T Thi Thanh Tam, P Thao Huong, D Dang Trung Nghia, H Tran Anh Vu, N Thi Hong Phuong, P Van Xang, N Dong, N Nhu Hiep, P Van Hung, N Tinh Hien, T Rabaa, M Thwaites, G Baker, S Van Tan, L van Doorn, H |
| author_sort | Thi Ty Hang, V |
| collection | OXFORD |
| description | Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription. |
| first_indexed | 2024-03-07T02:15:13Z |
| format | Journal article |
| id | oxford-uuid:a1fc0dcc-cb4f-4924-946f-91c7ba6b1c5e |
| institution | University of Oxford |
| last_indexed | 2024-03-07T02:15:13Z |
| publishDate | 2018 |
| publisher | F1000Research |
| record_format | dspace |
| spelling | oxford-uuid:a1fc0dcc-cb4f-4924-946f-91c7ba6b1c5e2022-03-27T02:17:13ZEvaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in VietnamJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a1fc0dcc-cb4f-4924-946f-91c7ba6b1c5eSymplectic Elements at OxfordF1000Research2018Thi Ty Hang, VThi Han Ny, NMy Phuc, TThi Thanh Tam, PThao Huong, DDang Trung Nghia, HTran Anh Vu, NThi Hong Phuong, PVan Xang, NDong, NNhu Hiep, PVan Hung, NTinh Hien, TRabaa, MThwaites, GBaker, SVan Tan, Lvan Doorn, HBackground: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription. |
| spellingShingle | Thi Ty Hang, V Thi Han Ny, N My Phuc, T Thi Thanh Tam, P Thao Huong, D Dang Trung Nghia, H Tran Anh Vu, N Thi Hong Phuong, P Van Xang, N Dong, N Nhu Hiep, P Van Hung, N Tinh Hien, T Rabaa, M Thwaites, G Baker, S Van Tan, L van Doorn, H Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam |
| title | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam |
| title_full | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam |
| title_fullStr | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam |
| title_full_unstemmed | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam |
| title_short | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam |
| title_sort | evaluation of the luminex xtag respiratory viral panel fast v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in vietnam |
| work_keys_str_mv | AT thityhangv evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT thihannyn evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT myphuct evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT thithanhtamp evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT thaohuongd evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT dangtrungnghiah evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT trananhvun evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT thihongphuongp evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT vanxangn evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT dongn evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT nhuhiepp evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT vanhungn evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT tinhhient evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT rabaam evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT thwaitesg evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT bakers evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT vantanl evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam AT vandoornh evaluationoftheluminexxtagrespiratoryviralpanelfastv2assayfordetectionofmultiplerespiratoryviralpathogensinnasalandthroatswabsinvietnam |