Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.

A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.

Bibliographic Details
Main Authors: Tanomsing, N, Imwong, M, Theppabutr, S, Pukrittayakamee, S, Day, N, White, N, Snounou, G
Format: Journal article
Language:English
Published: 2010
Description
Summary:A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.