Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis
In a discovery of the transfer of chloroplast biosynthesis activity to an inorganic material, ferredoxin-NADP+ reductase (FNR), the pivotal redox flavoenzyme of photosynthetic CO2 assimilation, binds tightly within the pores of indium tin oxide (ITO) to produce an electrode for direct studies of the...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
Royal Society of Chemistry
2017
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| _version_ | 1826288679952318464 |
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| author | Siritanaratkul, B Megarity, C Roberts, T Samuels, T Winkler, M Warner, J Happe, T Armstrong, F |
| author_facet | Siritanaratkul, B Megarity, C Roberts, T Samuels, T Winkler, M Warner, J Happe, T Armstrong, F |
| author_sort | Siritanaratkul, B |
| collection | OXFORD |
| description | In a discovery of the transfer of chloroplast biosynthesis activity to an inorganic material, ferredoxin-NADP+ reductase (FNR), the pivotal redox flavoenzyme of photosynthetic CO2 assimilation, binds tightly within the pores of indium tin oxide (ITO) to produce an electrode for direct studies of the redox chemistry of the FAD active site, and fast, reversible and diffusion-controlled interconversion of NADP+ and NADPH in solution. The dynamic electrochemical properties of FNR and NADP(H) are thus revealed in a special way that enables facile coupling of selective, enzyme-catalysed organic synthesis to a controllable power source, as demonstrated by efficient synthesis of l-glutamate from 2-oxoglutarate and NH4+. |
| first_indexed | 2024-03-07T02:17:21Z |
| format | Journal article |
| id | oxford-uuid:a2b8028d-f53e-46a5-823b-0b3f9a69f5f7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:17:21Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:a2b8028d-f53e-46a5-823b-0b3f9a69f5f72022-03-27T02:21:57ZTransfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a2b8028d-f53e-46a5-823b-0b3f9a69f5f7EnglishSymplectic Elements at OxfordRoyal Society of Chemistry2017Siritanaratkul, BMegarity, CRoberts, TSamuels, TWinkler, MWarner, JHappe, TArmstrong, FIn a discovery of the transfer of chloroplast biosynthesis activity to an inorganic material, ferredoxin-NADP+ reductase (FNR), the pivotal redox flavoenzyme of photosynthetic CO2 assimilation, binds tightly within the pores of indium tin oxide (ITO) to produce an electrode for direct studies of the redox chemistry of the FAD active site, and fast, reversible and diffusion-controlled interconversion of NADP+ and NADPH in solution. The dynamic electrochemical properties of FNR and NADP(H) are thus revealed in a special way that enables facile coupling of selective, enzyme-catalysed organic synthesis to a controllable power source, as demonstrated by efficient synthesis of l-glutamate from 2-oxoglutarate and NH4+. |
| spellingShingle | Siritanaratkul, B Megarity, C Roberts, T Samuels, T Winkler, M Warner, J Happe, T Armstrong, F Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis |
| title | Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis |
| title_full | Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis |
| title_fullStr | Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis |
| title_full_unstemmed | Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis |
| title_short | Transfer of photosynthetic NADP+/NADPH recycling activity to a porous metal oxide for highly specific, electrochemically-driven organic synthesis |
| title_sort | transfer of photosynthetic nadp nadph recycling activity to a porous metal oxide for highly specific electrochemically driven organic synthesis |
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